| Interaction between PⅡ and NifA in Azospirillum brasilense Sp7 |
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| 引用本文： | CHENSanteng DUJinping 等. Interaction between PⅡ and NifA in Azospirillum brasilense Sp7[J]. 科学通报(英文版), 2003, 48(2): 170-174. DOI: 10.1360/03tb9035 |
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| 作者姓名： | CHENSanteng DUJinping 等 |
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| 作者单位： | [1]DepartmentofMicrobiology.ChinaAgriculturalUniversity,Beijing100094,China [2]NationalKeyLab.forAgrobiotechnology,ChinaAgriculturalUniversity,Beijing100094,China |
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| 摘 要： | The interaction between PⅡ and NifA in A.brasilense Sp7 was investigated by using the yeast two-hybrid system.Our experimental results showed that PⅡ directly interacted with the entire NifA protein and its N-terminal domain,but did not interact with the central domin and the C-terminal domain of NifA.No interaction happened if glnB coding for PⅡ was frame-shift mutated.Pz,a homolog of PⅡ,had no interation with NifA.
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| 关 键 词： | 固氮菌 固氮基因 转录 激活子 PⅡ NifA 相互作用 |
| 收稿时间： | 2002-09-23 |
Interaction between PII and NifA in Azospirillum brasilense Sp7 |
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| Sanfeng?ChenEmail author,Jinping?Du,Lixian?Wu,Yinsuo?Zhao,Jilun?Li. Interaction between PII and NifA in Azospirillum brasilense Sp7[J]. Chinese science bulletin, 2003, 48(2): 170-174. DOI: 10.1360/03tb9035 |
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| Authors: | Sanfeng?Chen author-information" > author-information__contact u-icon-before" > mailto:chensf@mail.cau.edu.cn" title=" chensf@mail.cau.edu.cn" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,Jinping?Du,Lixian?Wu,Yinsuo?Zhao,Jilun?Li |
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| Affiliation: | (1) Department of Microbiology, China Agricultural University, 100094 Beijing, China;(2) National Key Lab. for Agrobiotechnology, China Agricultural University, 100094 Beijing, China |
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| Abstract: | The interaction between PinII and NifA in A. brasilense Sp7 was investigated by using the yeast two-hybrid system. Our experimental results showed that PinII directly interacted with the entire NifA protein and its N-terminal domain, but did not interact with the central domain and the C-terminal domain of NifA. No interaction happened if glnB coding for PinII was frame-shift mutated. Pz, a homolog of PinII, had no interation with NifA. |
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| Keywords: | Azospirillum brasilense yeast two-hybrid system PII NifA |
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