| 多因子正交试验对长春花离体培养条件的筛选 |
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| 引用本文： | 孙敏,伍春莲,汪洪,王颖. 多因子正交试验对长春花离体培养条件的筛选[J]. 西南师范大学学报(自然科学版), 2002, 27(2): 202-205 |
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| 作者姓名： | 孙敏 伍春莲 汪洪 王颖 |
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| 作者单位： | 西南师范大学生命科学学院,重庆,400715 |
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| 基金项目： | 重庆市科委攻关项目资助 |
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| 摘 要： | 应用正交设计法考察了植物生长物质、蔗糖对长春花愈伤组织诱导、丛生芽诱导和生根培养的影响。最佳愈伤组织诱导培养基为MS＋2，4－D0.8mg/L NAA 1mg/L ZT 0.2mg/L。丛生芽诱导最佳培养基为MS＋6－BA0.2mg L NAA 0.3mg/L＋蔗糖30g/L，最佳生根培养基为1／2MS＋NAA 0.2mg/L IBA 0.2mg/L 蔗糖30mg/L。
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| 关 键 词： | 多因子正交试验 长春花 离体培养条件 筛选 药用植物 |
| 文章编号： | 1000-5471(2002)02-0202-04 |
| 修稿时间： | 2001-09-14 |
Application of Orthogonal Design in Selecting Culture Condition in Vitro of Catharanthus Roseus |
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| SUN Min,WU Chun-lian,WANG Hong,WANG Ying School of Life Science,Southwest China Normal University,Chongqing ,China. Application of Orthogonal Design in Selecting Culture Condition in Vitro of Catharanthus Roseus[J]. Journal of southwest china normal university(natural science edition), 2002, 27(2): 202-205 |
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| Authors: | SUN Min WU Chun-lian WANG Hong WANG Ying School of Life Science Southwest China Normal University Chongqing China |
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| Affiliation: | SUN Min,WU Chun-lian,WANG Hong,WANG Ying School of Life Science,Southwest China Normal University,Chongqing 400715,China |
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| Abstract: | Catharanthus roseus (L.) G. Don is an important pharmaceutical plant against cancer, including many kinds of alkaloids. This article studies on the callus inducing and emphatically discuss foundation of culture system of Catharanthus roseus. Effects of plant hormone and sucrose on the tufted-bud induction and the tufted-bud rooting culture of Catharanthus roseus were studied by orthogonal design L-9(3+4). The best media of callus inducing ,bud inducing and root inducing are MS+2,4-D 0.8 mg/L+NAA 1mg/L+ZT 0.2 mg/L, MS+6-BA 0.2 mg/L+NAA 0.3 mg/L, 1/2 MS+NAA 0.2 mg/L+IBA 0.2 mg/L respectively. |
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| Keywords: | Catharanthus roseus orthogonal design culture condition |
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