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广西四川金矿床区土壤样品中蜡状芽孢杆菌的分离和鉴定
引用本文:汤显春,陈绳亮,罗勤,熊克娟,谢树成,周修高.广西四川金矿床区土壤样品中蜡状芽孢杆菌的分离和鉴定[J].华中师范大学学报(自然科学版),1999,38(2):0-0.
作者姓名:汤显春  陈绳亮  罗勤  熊克娟  谢树成  周修高
作者单位:中国科学院武汉病毒研究所!武汉,430071,中国科学院武汉病毒研究所!武汉,430071,中国科学院武汉病毒研究所!武汉,430071,中国科学院武汉病毒研究所!武汉,430071,中国地质大学武汉地球科学学院!武汉,430074,中国地质大学武汉地球科学学院!武汉,430074
基金项目:地矿部生物成矿作用重点学科生长点基金,湖北省科委重点项目
摘    要:从广西金芽、四川阿西所属的三个金矿区采集了土壤样品55份,采用选择性培养基从10~(-2)稀释度的平皿中分离到阳性菌739株,从中挑取11株,其分出率为1.5%,并对分离株进行了光镜和电镜形态学观察,其大小为1~1.4×2.5~3.5μm;芽孢椭圆形.中生,没有孢囊和伴孢晶体,并对11株分离株的生理生化特性进行了测定,结果表明,除C_(17)分离株V、P反应阴性有待进一步分析外,其余10株都与标准株完全相同,因此,应归属芽孢杆菌属蜡状芽孢杆菌(Bacilluscereus).

关 键 词:金矿区  土壤样品  蜡状芽孢杆菌  分离  鉴定
收稿时间:1999-02-25

Isolation and identification of Bacillus cereus from soil samples of gold deposit in Guangxi and Sichuan
Tang Xianchun,Chen Shenliang,Luo Qin,Xiong Kejian.Isolation and identification of Bacillus cereus from soil samples of gold deposit in Guangxi and Sichuan[J].Journal of Central China Normal University(Natural Sciences),1999,38(2):0-0.
Authors:Tang Xianchun  Chen Shenliang  Luo Qin  Xiong Kejian
Abstract:The isolation and identification of Bacillus cereus from soil samples ofgold deposit in Guangxi and Sichuan Provinces were reported. Fiftyfive soil sampleswere collected from 3 deposits. A total of 739 Positive colonies were isolated byEYA selective medium and 11 isolates were picked out from the colonies, the rateof isolation is 1. 5%. The morphology of cells and spores of these strains was inves-tigated under light and electron-microscope. The cells are Grampositive rods, 1. 0to 1. 4 pm by 2. 5 to 3. 5 pm, The spores are elliptical, central and without sporani-um and parasporal crystal. The physiological and biochemical characteristics of 11strains were examined. The results indicated that 10 of them should be identified asBacillus cereus
Keywords:gold deposit  soil samples  Bacillus cereus  isolation  identification
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