| Fluorescence Determination of Artemisinin Using Hemoglobin as Catalyst and Pyronine B as Substrate |
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| 引用本文： | CHEN Lihua~ 1,2,ZHANG Yongkang~1,YIN Hong~1,LIU Liuzhan~2,YANG Zhaoxia~1,SHEN Hanxi~2 1. College of Chemistry and Chemical Engineering,Jishou University,Jishou 416000,Hunan,China , 2. College of Chemistry,Nankai University,Tianjin,300071,China. Fluorescence Determination of Artemisinin Using Hemoglobin as Catalyst and Pyronine B as Substrate[J]. 武汉大学学报:自然科学英文版, 2006, 11(3): 704-708. DOI: 10.1007/BF02836694 |
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| 作者姓名： | CHEN Lihua~ 1 2 ZHANG Yongkang~1 YIN Hong~1 LIU Liuzhan~2 YANG Zhaoxia~1 SHEN Hanxi~2 1. College of Chemistry and Chemical Engineering Jishou University Jishou 416000 Hunan China 2. College of Chemistry Nankai University Tianjin 300071 China |
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| 作者单位： | CHEN Lihua~ 1，2，ZHANG Yongkang~1，YIN Hong~1，LIU Liuzhan~2，YANG Zhaoxia~1，SHEN Hanxi~2 1. College of Chemistry and Chemical Engineering，Jishou University，Jishou 416000，Hunan，China ; 2. College of Chemistry，Nankai University，Tianjin，300071，China |
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| 基金项目： | 国家高技术研究发展计划(863计划);湖南省教育厅资助项目 |
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| 摘 要： | 0 IntroductionMaalnadria siusb atr ompajicosr .he aAltrthe pmrisoibnlienmi (n qtihneghtraoopsiucs,QHS,Fig.1) is a sesquiterpene endoperoxide isola-ted fromArtemisia annuaL., an ancient Chineseherbal medicine usedfor treatment of fever and ma-laria.Studies of the structure and activity relation-ship have shownthat endoperoxide groupis essentialfor anti malarial activity of QHS and absence of thismoiety lead to completely loss in activity of thedrug. Many techniques have been developed to de…
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| 关 键 词： | 血红蛋白 派若宁B 碱性染料 荧光减少率 测定 催化剂 酶解物 |
| 文章编号： | 1007-1202（2006）03-0704-05 |
| 收稿时间： | 2005-04-26 |
Fluorescence determination of artemisinin using hemoglobin as catalyst and pyronine B as substrate |
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| Chen Lihua,Zhang Yongkang,Yin Hong,Liu Liuzhan,Yang Zhaoxia,Shen Hanxi. Fluorescence determination of artemisinin using hemoglobin as catalyst and pyronine B as substrate[J]. Wuhan University Journal of Natural Sciences, 2006, 11(3): 704-708. DOI: 10.1007/BF02836694 |
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| Authors: | Chen Lihua Zhang Yongkang Yin Hong Liu Liuzhan Yang Zhaoxia Shen Hanxi |
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| Affiliation: | (1) College of Chemistry and Chemical Engineering, Jishou University, 41600 Jishou, Human, China;(2) College of Chemistry, Nankai University, 300071 Tianjin, China |
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| Abstract: | Fluorescence decrease ratio was applied to determine of artemisinin (qinghaosu, QHS) based on the catalytic effect of hemoglobin (Hb) using tetraethyldiaminoxanthenyl chloride (Pryonine B, PB) as monitor in this work. Experimental result show that the catalytic characteristics of Hb for QHS, being expressed as Michaelis-Menten parameters,K m,V max, andK cat are 2.8×10−5 mol ·L−1, 4.2×106 mol · L−1 · s−1 and 280 s−1, respectively. Like hemin, enzymatic bioactivities of Hb is inhibited by both deactivated agents and high temperature where-as enhanced by ethanol. With the catalytic action of Hb, quantitative method of QHS can be established based on the fluorescence decrease of PB and linear relationship between the fluorescence decrease ratioF 0/F and the concentration of QHS is in the range of (0.0−1.1)×105 mol · L−1 with detection limit (3σ) being 7.2 ×10−9 mol ·L−1. The concentration of QHS in the media of plasma or urine was detected using this method. Foundation item: Supported by the National Natural Science Foundation of China (20075012) and the Scientific Research Foundation of Human Education Committee (02C313) Biography: CHEN Lihua (1961-), female, Ph. D., Associate professor, research direction: analytical chemistry |
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| Keywords: | hemoglobin artemisinin pyronine B fluorescence decrease ratio |
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