A practicable variant of the ion exchange method for the radiometric estimation of ornithine decarboxylase activity

A known ornithine decarboxylase assay working with ion exchange separation of 3H]ornithine and 3H]putrescine has been revised. The assay can be performed in disposable 1.5 ml vessels with a total of four pipetting steps. The separation of enzyme substrate and product, respectively, requires 3 h per 50 samples. The detection limit is about 50 pmoles 3H]putrescine formed.