陆梁油田菌群结构解析及激活后群落变化

 针对新疆陆梁油田呼图壁河组注水井LU3064 注入水，设计一组营养剂激活配方，该配方能够成功乳化原油。经平板计数法、最大或然数计数法（MPN）和理化性质检测发现，激活后注入水菌浓明显增加，表面张力下降37%。采用建立细菌16S rDNA克隆文库方法，对LU3064 注入水、LU3036 采出液、LU3064 激活液细菌进行多样性研究。从克隆文库中随机挑选100 个克隆子测序，并在GenBank 中比对，结果表明，注入水菌群具有高度多样性，优势菌属于Alphaproteobacteria；采出液较地层水菌群结构简单，存在Pseudomonas（假单胞菌属）、Bacillus（芽孢杆菌属）等功能菌属；激活液菌群结构最为单一，仅存在Bacillus（芽孢杆菌属）。Bacillus 在注入水和采出液中均有发现，但都不是优势菌种。该激活剂配方成功激活了可在地层中生长的功能菌属Bacillus。

Microbial Community and Community Variability after Activated in Luliang Oilfield

A group of nutrient activators with respect to injection well LU3064 in Xinjiang oil field Hutubi river group are designed, for emulsifying the crude oil. With the bacteria counting method, the MPN method, and the physiochemical property detection method, it is discovered that, in the injection water, upon activation, the concentration of bacteria rises significantly, while its surface tension decreases by 37%. With the molecular biological techniques (16S rDNA clone library), the LU3064 injection water, the LU3036 produced water and the LU3064 activated water are studied. One hundred clones are randomly selected from the clone library to blast in Genbank. The observations show that the injection water is highly diversified, and Alphaproteobacteria is the dominating bacteria. Compared to the injection water, the produced water has a simpler structure, which provides habitats with function bacteria genera such as Pseudomonas, Bacillus, etc. The community structure is found most monotonous in the activation fluid, only Bacillus can be found. Bacillus is discovered in both the injection water and the produced water, but does not dominate in them. The formula successfully activates Bacillus which can be grown in the stratum.