乳腺癌患者血浆和尿液的1H-NMR代谢组学

 为探讨乳腺癌患者血浆、尿液代谢组学变化，建立早期诊断的代谢标志物体系。选取乳腺癌患者45例，健康对照30例，获得血浆1H-NMR谱图，对所得自由衰减信号进行分段积分、数据归一化后用正交偏最小二乘法判别分析（OPLS-DA）进行模式识别。与健康人群相比，乳腺癌患者血浆中多种氨基酸含量下降，包括亮氨酸、缬氨酸、苯丙氨酸、酪氨酸、丙氨酸、组氨酸、甘氨酸、脂类（LDL）、β-葡萄糖、乳酸、胆碱、肉碱、肌醇显著减少，差异具有统计学意义（P<0.05）；血浆糖蛋白、丙二酸、乙酰乙酸、β-羟丁酸、丙酮显著增多，差异具有统计学意义（P<0.05）。与健康人相比，乳腺癌患者尿液中异亮氨酸、缬氨酸、谷氨酸、组氨酸、二甲胺、马尿酸、甜菜碱、牛磺酸、琥珀酸、柠檬酸、肌酸、肌酸酐减少，差异具有统计学意义（P<0.05）；乳酸增多，差异具有统计学意义（P<0.05）。通过乳腺癌患者的代谢组学的变化，建立可以用于乳腺癌早期诊断的血浆、尿液代谢标志物体系。

Metabonomics Studies on Plasma and Urine of Patients with Breast Cancer Using NMR Spectroscopy

Metabonomics studies on plasma and urine of patients with breast cancer using NMR spectroscopy are to establish metabolic markers correlated with breast cancer to guide clinic diagnosis and provide the new ideas and methods. In this study, a total of 45 patients with breast cancer were enrolled and 50 healthy volunteers as controls. Meanwhile, blood and urine samples from the subjects were collected by the researchers for testing. For each sample, the 1H nuclear magnetic resonance (NMR) based metabonomic analysis was performed, the subsection integral of the free attenuation signal was computed, and the obtained data were analyzed by the orthogonal partial least-squares discriminant analysis (OPLS-DA). The results of the study show that compared with the healthy people, leucine, valine, phenylalanine, tyrosine, alanine, histidine, and glycine are significantly decreased (P<0.05); lipid (LDL), beta glucose，lactic acid, choline, inositol and creatine are significantly reduced (P<0.05); glycoprotein, malonic acid, acetoacetic acid, beta hydroxybutyric acid, and acetone are increased significantly (P<0.05) in the plasma of patients with breast cancer. Moreover, isoleucine, valine, glutamic acid, histidine, dimethylamine, hippuric acid, betaine, taurine, succinic acid, citric acid,creatine, and creatinine are decreased (P<0.05) and Lactic acid increased (P<0.05) in urine of patients with breast cancer. Through this study, metabolic markers correlated with breast cancer using changes of metabonomics in plasma and urine have been established.