CTMAB催化增敏分光光度法测定食品中的己烯雌酚

利用CTMAB（三甲基十六烷基溴化铵）具有相转移催化和增敏络合体系等特性，建立了用可见分光光度法测定己烯雌酚（DES）的方法。研究表明，DES能将Fe（Ⅲ）还原成Fe（Ⅱ），Fe（Ⅱ）与k[Fe（CN）61在CTMAB存在的环境下形成稳定的多元胶束络合体系，其最大吸收波长在736nm处。DES的质量浓度在0．03256～0．6521ug／mL范围内与吸光度呈良好线性关系，线性回归方程A=0．0027＋0．3990P（μg／mL），相关系数R2=0．9991，摩尔吸光系数s=5．43×105（L·mol-1·cm-1），检出限1．35μg／mL，相对标准偏差为0．89％，方法用于测定奶粉中DES含量，回收率为89-3％～101．6％。

Determination of Diethylstilbestrol in Food by Catalytic and Sensitive Spectrophotometry with CTMAB

A catalytic and sensitive spectrophotometric method for the determination of diethyl- stilbestrol（DES） with CTMAB has been developed. The method is based on the phase transfer cataly- sis and sensitive complex ation characteristic of CTMAB. The study showed that Fe （ Ⅱ） was re- duced to Fe （ Ⅱ ） by DES and a stable multi-micelle complex was formed by Fe （Ⅱ ） and K3 [Fe （CN）6] in the existence of CTMAB, the maximum absorption is at at 736 nm. Lambert-Beer law was well obeyed at DES concentrations ranging from 0. 032 56 to 0. 652 1 μg/mL, regression equation was A = 0. 002 7＋0. 399 0 p , correlation coefficient R2 was O. 999 1, detection limit was 1.35 μg/mL and the molar absorption coefficient was ε = 5.43 ×105（L. mol-1· cm-1） ; the relative standard devia- tion was 0.89%. The proposed method was used in the determination of DES in powdered milk and the recovery was 89.3% ~ 101.6%.