| 一种放线酮化合物含量的几种测定方法比较 |
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| 引用本文： | 杨波,李铭刚,夏振远,赵江源,李一青,郭端强,文孟良. 一种放线酮化合物含量的几种测定方法比较[J]. 云南大学学报(自然科学版), 2006, 0(Z1) |
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| 作者姓名： | 杨波 李铭刚 夏振远 赵江源 李一青 郭端强 文孟良 |
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| 作者单位： | 云南大学云南省微生物研究所 云南省烟草科学研究院 云南大学云南省微生物研究所 云南昆明 云南昆明 云南玉溪 |
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| 摘 要： | 分别采用生物测定法、分光光度法和高效液相色谱法(HPLC)检测云南放线酮(4-[2-(3,5-Dimethyl-2-oxo-cyclohexyl)-2-hydroxy-ehthy]-piperidine-2,6-dione)的含量,结果表明在这3种方法中,生物测定法灵敏度较后二法低,但能反应真实的生物学效应,并具有不需特殊设备,操作简便的特点,适用于处理样品量较多的定性和定量测定;分光光度法检测速度快、检测灵敏度较高,适用于中等样品量的定量测定;而HPLC法检测准确、灵敏度最高,则适用于样品量较少的精确定量测定.
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| 关 键 词： | 放线酮 生物测定法 分光光度法 高效液相色谱法 |
Comparison with the determination of actidione by different methods |
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| YANG Bo,LI Ming-gang,XIA Zhen-yuan,Zhao Jiang-yuan,Li Yi-qing,Guo Duan-qiang,WEN Meng-liang. Comparison with the determination of actidione by different methods[J]. Journal of Yunnan University(Natural Sciences), 2006, 0(Z1) |
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| Authors: | YANG Bo LI Ming-gang XIA Zhen-yuan Zhao Jiang-yuan Li Yi-qing Guo Duan-qiang WEN Meng-liang |
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| Affiliation: | YANG Bo~1,LI Ming-gang~1,XIA Zhen-yuan~2,Zhao Jiang-yuan~1,Li Yi-qing~1,Guo Duan-qiang~1,WEN Meng-liang~1 |
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| Abstract: | The microbiological assay method,UV spectrophotometry method and HPLC method were selected to determine the content of Yunnan-actidione(4-[2-(3,5-Dimethyl-2-oxo-cyclohexyl)-2-hydroxy-ehthy)-piperidine-2,6-dione).The result indicated that the microbiological assay method,because of its high sensitivity,no need of special equipment and easy to handle,is suitable for the qualitative analysis and quantitative determination for large amount samples;The UV spectrophotometry method,because of its fast testing speed and higher sensitivity,can be used to assay the middle amount of samples.However,The HPLC assay method,because of its the highest sensitivity,can be used to assay the small amount of samples precisely and quantitatively. |
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| Keywords: | actidione microbiological assay UV spectrophotometry HPLC |
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