| 金纳米颗粒修饰的固定化葡萄糖氧化酶酶膜制备 |
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| 引用本文： | 毕春元,周万里,赵晓华,朱思荣,史建国,崔精诚,陈皓,郭长苗,冯凯,栗兵,陈晓.金纳米颗粒修饰的固定化葡萄糖氧化酶酶膜制备[J].山东科学,2010,23(2):14-17. |
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| 作者姓名： | 毕春元 周万里 赵晓华 朱思荣 史建国 崔精诚 陈皓 郭长苗 冯凯 栗兵 陈晓 |
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| 作者单位： | 1. 山东省科学院生物研究所，山东 济南 250014， 2. 山东大学化学院， 山东 济南 250100 |
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| 摘 要： | 分别利用柠檬酸钠和NaBH4作为还原剂、以PVP作为包覆剂制备不同类型、不同包覆情况的金纳米粒子,并用紫外可见分光光谱仪、透射电微镜等手段对制备的金纳米粒子的粒径、形貌和分散性进行表征。用金纳米粒子对葡萄糖氧化酶膜进行修饰,并在SBA生物传感分析仪上进行感应测量。研究结果表明,粒径小、分散性好的球状金纳米粒子可以极大地提高葡萄糖酶膜的灵敏度。
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| 关 键 词： | 金纳米粒子 葡萄糖氧化酶 固定化酶膜 生物传感器 |
The Preparation of Gold Nanoparticles Modified Innobilized Glucose Oxidase Membranes |
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| BI Chun-yuan,ZHOU Wan-li,ZHAO XIAO-hua,ZHU Si-rong,SHI Jian-guo,CUI Jing-cheng,CHEN hao,GUO Chang-miao,FENG Kai,LI Bing,CHEN Xiao.The Preparation of Gold Nanoparticles Modified Innobilized Glucose Oxidase Membranes[J].Shandong Science,2010,23(2):14-17. |
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| Authors: | BI Chun-yuan ZHOU Wan-li ZHAO XIAO-hua ZHU Si-rong SHI Jian-guo CUI Jing-cheng CHEN hao GUO Chang-miao FENG Kai LI Bing CHEN Xiao |
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| Institution: | 1. Biology Institute of Shandong Academy of Science, Jinan 250014, China; 2. School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China |
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| Abstract: | We prepared different gold nanoparticles with sodium citrate,NaBH4(reducing agents) and PVP(capping agent).We employed an ultraviolet-visible spectroscopy and a transmission electron microscopy to characterize their size,morphology and dispersibility.We also modified the glucose oxidase membrane electrodes with these gold nanoparticles and measured the responding signals with a SBA biosensor.Results show that spherical small size and good dispersibility gold nanoparticles can greatly improve the sensitivity... |
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| Keywords: | gold nanoparticles glucose oxidase immobilized membrane biosensor |
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