紫锥菊叶柄高效再生体系的建立

紫锥菊具有增强免疫和抗感染的作用，在国际上具有悠久的应用历史，近年来国内也有引种. 该文以紫锥菊的不同器官（根、叶片、叶柄）为外植体，研究各激素配比对不同外植体建立再生体系的影响. 〖JP2〗结果表明：根、叶片、叶柄不定芽分化的适宜培养基分别为MS+05 mg/L 6-BA+02 mg/L NAA、MS+20 mg/L 6-BA、〖JP〗MS+05 mg/L 6-BA，诱导率分别为438%、737%和948%；增殖的适宜培养基为MS+05 mg/L 6-BA，增殖系数为90，生根培养基为MS基本培养基. 研究结果为紫锥菊的快速繁殖和进一步开展遗传转化奠定基础.

Establishment of An Efficient Regeneration System for Echinacea Purpurea Petiol

Echinacea Purpurea, which has the functions of enhancing immunity and anti-infection, has been used as a medicinal plant for a long history worldwide, and was introduced to China in recent years. In order to establish an efficient regeneration system of E. Purpurea, we studied the effect of concentrations of 6-BA and NAA on the plant regeneration of different explants. The results showed that: a, the optimum media for bud differentiation from petiol, leaf and root were MS+05 mg/L 6-BA+02 mg/L NAA, MS+20 mg/L 6-BA, and MS+05 mg/L 6-BA, respectively; b, the optimum media for differentiation and rooting were MS+05 mg/L 6-BA, MS, respectively; c, the multiplication coefficient reached to 90. This work provides a practical plant regeneration method for rapid propagation and genetic modification of E.Purpurea.