| 产胞外木聚糖酶青霉菌发酵条件的正交设计试验 |
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| 引用本文： | 毕瑞明,任少亭,高庆义,孙迅.产胞外木聚糖酶青霉菌发酵条件的正交设计试验[J].河北大学学报(自然科学版),1999(3). |
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| 作者姓名： | 毕瑞明 任少亭 高庆义 孙迅 |
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| 作者单位： | 菏泽师范专科学校生物系!山东菏泽274015 |
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| 摘 要： | 正交设计试验结果表明，青霉菌（Ｐｅｎｉｃｉｌｌｉｕｍ ｓｐ．ｍ８） 胞外木聚糖酶活力达９２．９６ Ｕ／ｍＬ，合适的产酶发酵条件为：培养基（ｇ／Ｌ），麦草粉４０，（ＮＨ４）２ＳＯ４ ４．４４，ＫＨ２ＰＯ４ １．０，ＭｇＳＯ４ ·７Ｈ２Ｏ０．５，ＮａＣｌ０．３，Ｔｗｅｅｎ ８０ ３．０，ＣａＣＯ３ １．０；每亳升培养基接种２．０×１０５ 个孢子，２８ ℃，水浴振荡培养４ ｄ（１２０ｒ／ｍｉｎ）。
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| 关 键 词： | 胞外木聚糖酶 青霉菌(Penicilliumsp.m8) 发酵 正交试验 |
An Orthogonal Experiment in the Fermentation of Penicillium sp. Producing Extracellular Xylanases |
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| BI Rui_ming,REN Shao_ting,GAO Qing_yi,SUN Xun.An Orthogonal Experiment in the Fermentation of Penicillium sp. Producing Extracellular Xylanases[J].Journal of Hebei University (Natural Science Edition),1999(3). |
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| Authors: | BI Rui_ming REN Shao_ting GAO Qing_yi SUN Xun |
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| Abstract: | An orthogonal experiment was carried out for optimization of fermentation of Penicillium sp. m8 in order to produce extracellular xylanases. The xylanase activity of culture filtrate of m8 was 92.96 U/mL. The medium(g/L) contained wheat straw powder 40,(NH4)2SO4 4.44, KH2PO4 1.0,MgSO4·7H2O 0.5,NaCl 0.3,Tween 80 3.0, CaCO3 1.0. 1.0×107 spores were inoculated into 50 mL medium in 250 mL Erlenmeyer Flask in a water rocker(120 r/min,28 ℃)which stirred for 4 days. |
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| Keywords: | extracellular xylanases Penicillium sp m8 fermentation orthogonal experiment |
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