Fehlende Wasserstoff-Übertragung von Östradiol auf Androstendion im Diäthylnitrosamin-induzierten Hepatom der Ratte

Summary In liver-tissue of female rats estradiol-17 -T is oxydized by NAD or NADP, yielding the tritiumlabelled coenzymes NAD-T or NADP-T which can transfer the tritium to androstendione. So tritium-labelled androstandiol is obtained. Of the added activity 10–30% is oxydized to water. In diethylnitrosamine-induced hepatomas estradiol-17 -T is oxydized to tritium-water in a comparatively small amount and no transport of the tritium to reduction-products of androstendions occurs. Testing the in-vivo-oxydation of estradiol in normal female rats and in females with hepatoma with the aid of the Stoffwechsel-labil labelling (measuring the specific activity of tritium in body-water after injecting estradiol-17 -T) a lower oxydation-rate was also found in rats with hepatoma than in normal rats.