不同粒径马钱子粉中马钱子碱、士的宁的含量分析与比较

为考察粒径对马钱子粉中活性(毒性)成分含量的影响,制备了20～200目下的马钱子粉样品;采用扫描电子显微镜(SEM)观察各样品的微观形貌,利用薄层色谱法(TLC)对各样品进行定性鉴别并用高效液相色谱法(HPLC)测定各样品中马钱子碱、士的宁含量。结果表明:不同粒径下马钱子粉样品微观形态组成比例存在差异,而且马钱子碱、士的宁含量不同,马钱子碱(或士的宁)在部分样品中的含量存在显著性差异。因此在马钱子药粉入药时,应严格控制其粒径,结果显示宜将马钱子粉的粒径控制在100～200目之间。

Analysis and Comparison of Content Dependence of Brucine and Strychnine on Different Particle Sizes of Semen Strychni Powder

To study the effect of the particle size on the active(toxic) component contents of Semen Strychni powder,20—200 mesh of Semen Strychni powder samples were prepared.The micro morphology of these samples were characterized by scanning electron microscope(SEM) and the samples were qualitatively identified by TLC.The contents of brucine and strychnine of each sample were determined by HPLC.The results indicated that there existed some differences between morphology compositions of Semen Strychni powder samples with different particle sizes.Moreover,the contents of brucine,strychnine of different samples were different,and the contents of brucine(or strychnine) in some samples of Semen Strychni powder were significantly different.So when Semen Strychni powder is applied to traditional Chinese medicine,its particle size should be highly controlled.Upon the results of the research,the particle size of Semen Strychni powder had better be 100—200 mesh.