过氧化氢对拟南芥生长素信号传导相关蛋白的影响

本论文通过添加外源过氧化氢来探究其对植物生长素信号的影响。以GUS基因作为报告基因，研究拟南芥中各生长素相关蛋白(生长素含量标志蛋白DR5、输入蛋白AUX1、输出蛋白PIN1和PIN2)对过氧化氢的响应。结果表明过氧化氢处理后拟南芥呈植株变小、根变短和不定根数目增多的表型；与对照相比，GUS染色后各生长素相关蛋白的表达下降，这表明过氧化氢对植物生长素的合成与运输起一定的抑制作用。在过氧化氢处理的基础上，添加抗氧化物质发现植株表型有明显的恢复，GUS染色也表明相关蛋白的表达量均增加；另外，0.0001 ?mol/L的外源IAA也能恢复过氧化氢处理后拟南芥的表型和生长素相关蛋白的表达。综上所述，过氧化氢主要通过抑制植物体内的生长素信号来调控植物的生长发育。

Study of Hydrogen Peroxide Effects on The Auxin Signal in Plants

The effect of exogenous hydrogen peroxide on the auxin signal is studied. The GUS was regarded as the reporter gene to research how the auxin signaling proteins, including DR5,a maker of auxin level;AUX1,the auxin input protein; PIN1 and PIN2, auxin output proteins, response to the effects of hydrogen peroxide. The results showed that Arabidopsis thalianas displayed smaller size, shorter root and increased adventitious root number after hydrogen peroxide treatment. The GUS staining results showed that the expression of auxin-related proteins decreased, and suggested that hydrogen peroxide can inhibit the auxin synthesis and transport. Based on the effect of hydrogen peroxide, some antioxidant substances were added to the medium, and the result showed that the phenotype of Arabidopsis and the GUS staining were rescued obviously. Moreover, 0.0001 ?mol/L IAA also could rescue the phenotype of Arabidopsis and the expression of auxin proteins. All the results suggest that the hydrogen peroxide can regulate the plant growth and development by inhibiting the auxin signal.