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转基因pYBGAl酵母发酵木质纤维素水解液制备燃料乙醇
引用本文:梁鲜香,吉田孝,金日光.转基因pYBGAl酵母发酵木质纤维素水解液制备燃料乙醇[J].北京化工大学学报(自然科学版),2009,36(z1):100-103.
作者姓名:梁鲜香  吉田孝  金日光
作者单位:北京化工大学,化工资源有效利用国家重点实验室,北京,100029;日本北见工业大学生物环境化学科,生物高分子化学研究室,日本,北见,0900081
摘    要:采用转基因pYBGA1酵母发酵葡萄糖及纤维二糖培养基,通过交叉实验研究了温度、酵母投入量对乙醇产率的影响;对木质纤维素进行爆破预处理,得到葡萄糖和纤维二糖的质量分数占50%预处理水解液,采用转基因pYBGA1酵母对预处理水解液发酵制备乙醇。结果表明,转基因pYBGA1酵母最佳发酵温度为28℃,最佳酵母投入量为108mL-1;当水解液中糖浓度为20g/L时,预处理水解液发酵得到乙醇的最大浓度可达6.8g/L。

关 键 词:木质纤维素  生物转化  燃料乙醇  发酵
收稿时间:2009-05-19

Ethanol production from lignocellulose hydrolysate by yeast-pYBGA1
LIANG XianXiang,YOSHIDA Takashi,JIN RiGuang.Ethanol production from lignocellulose hydrolysate by yeast-pYBGA1[J].Journal of Beijing University of Chemical Technology,2009,36(z1):100-103.
Authors:LIANG XianXiang  YOSHIDA Takashi  JIN RiGuang
Institution:1. State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China; 2. Biological Research Center of Polymer Chemistry, Kitami Institute of Technology, Kitami 0900081, Japan
Abstract:he glucose and cellobiose was fermented by using pYBGA1 recombinant yeast, study the effects of the temperature and concentration of yeast-pYBGA1 on the ferment ethanol rate by using cross experiments; the lig-nocellulosic was pretreated through steam explosion pretreatment method, the glucose and cellobiose content in the pretreatment hydrolysate was 50 % ; the pretreatment hydrolysate was fermented by using yeast-pYBGA1 to prepare the ethanol. Results shows that, the best fermentation temperature was 28℃ and the best to the ethanol productivity was 10~8 mL~(-1) ; the highest concentration of ethanol by direct fermentation of mixed sugar in ligno-cellulose-pretreatment hydrolysate is 6.8 g/L when the suger concentration is 20 g/L in the hydrolysate.
Keywords:lignocellulosic  bioconversion  bio-ethanol  fermentation
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