IS4 peptide forms ion channel in rat skeletal muscle cell membrane |
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| Authors: | Lin Bao Zhenwei Miao Pei’ai Zhou Yun Jiang Renji Zhang Youqi Tang |
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| Affiliation: | (1) State Key Laboratory of Biomembrane and Membrane Biotechnology, Peking University, 100871 Beijing, China;(2) College of Life Sciences, Peking University, 100871 Beijing, China;(3) State Key Loboratory for Structural Chemistry of Unstable and Stable Species, Peking University, 100871 Beijing, China |
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| Abstract: | A 22-mer peptide, identical to the primary sequence of domain I segment 4 (IS4) of rat brain sodium channel I, has been synthesized. IS4 peptide can incorporate into cultured rat skeletal myotube membranes and form ion channels. With patch clamp cell-attached technique single channel currents through IS4 channels can be recorded. The single channel conductances of IS4 channels are distributed heterogeneously. With different holding potentials, the mean open time, the mean closed time and the mean open probability are different respectively. IS4 channels are selective for Na+, Li+ and K+, but not for Cl−. |
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| Keywords: | synthetic IS4 peptide cation selective channel rat skeletal myotube |
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