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| 液相色谱-串联质谱法测定血浆中丹参素浓度 | |
| 引用本文： | 盖芸芸,沙春洁,刘万卉.液相色谱-串联质谱法测定血浆中丹参素浓度[J].烟台大学学报(自然科学与工程版),2008,21(1):35-39. |
| 作者姓名： | 盖芸芸 沙春洁 刘万卉 |
| 作者单位： | 1. 烟台大学,药学院,山东,烟台,264005 2. 山东绿叶制药有限公司,山东,烟台,264005 3. 烟台大学,药学院,山东,烟台,264005;山东绿叶制药有限公司,山东,烟台,264005 |
| 摘 要： | 研究了液相色谱-串联质谱法测定血浆中丹参素含量的方法．在实验确定的样品处理及液相色谱-质谱测定条件下，丹参素在2-400ng·mL。范围内呈良好的线性关系，加权最小二乘法计算得r为0．9985，最低定量限为2ng·mL^-1；血浆中加入丹参素浓度为5．0、50．0和400．0ng·mL“时的绝对回收率分别为54．3％、55．2％和51．6％，准确度（RE％）分别为-9．90％、-5．42％和4．50％，日内、日间精密度（RSD）分别为5．57％、5．54％、6．36％和2．86％、6．22％、9．02％．该方法灵敏度高、精密度和准确度好，满足药代动力学研究的要求． |
| 关 键 词： | 丹参素 血药浓度 液相色谱-串联质谱法 |
| 文章编号： | 1004-8820（2008）01-0035-05 |
| 收稿时间： | 2007-03-10 |
| 修稿时间： | 2007年3月10日 |
Liquid Chromatography-Tandem Mass Spectrometric Method for Determination of Danshensu in Plasma |
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| GAI Yun-yun,SHA Chun-jie,LIU Wan-hui.Liquid Chromatography-Tandem Mass Spectrometric Method for Determination of Danshensu in Plasma[J].Journal of Yantai University(Natural Science and Engineering edirion),2008,21(1):35-39. | |
| Authors: | GAI Yun-yun SHA Chun-jie LIU Wan-hui |
| Institution: | GAI Yun-yun, SHA Chun-jie , LIU Wan-hui (1. School of Pharmacy, Yantai University, Yantai 264005, China; 2. Shandong Luye Pharmacentical Company, Yantai 264003, China) |
| Abstract: | A sensitive liquid chromatography-tandem mass spectrometric （LC/MS/MS） method for assaying Danshensu in dog plasma was developed and fully validated. With the optimized LC/MS/MS and sample preparation method, the calibration curves show a good linearity over the range of 2 -400 ng·mL^-1. Fitted with a weighted linear least-squares regression analysis, the linear regression （r） is 0.9985. The method is validated at three concentrations of 5.0,50 and 400 ng·mL^-1 of Danshensu. The intra- and inter-day precision is 5.57%,5.54%, 6.36% and 2.86%, 6.22% 9.02%, the extract recovery is 54.3%, 55.2% and 51.6%, and the accuracy （ RE% ） is - 9.90%, - 5.42 % and 4.50 %, respectively. The assay is sensitive, accurate and suitable for pharmacokinetic study of Danshensu in plasma. |
| Keywords: | Danshensu concentration in blood LC/MS/MS |
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