| A perspective on fish gonad manipulation for biotechnical applications |
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| 引用本文： | HU wei WANG Yaping ZHU Zuoyan. A perspective on fish gonad manipulation for biotechnical applications[J]. 科学通报(英文版), 2006, 51(1): 1-7. DOI: 10.1007/s11434-005-1055-3 |
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| 作者姓名： | HU wei WANG Yaping ZHU Zuoyan |
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| 作者单位： | State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan 430072, China |
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| 基金项目： | Acknowledgements This work was financially supported by the National Natural Science Foundation of China （Grant No. 30430540 ＆ 30130050）, the ＂863＂ High Technology Project （Grant No. 2004AA213121） and the Development Plan of the State Key Fundamental Research of China （Grant No. 2001 CB 109006）. |
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| 摘 要： | Fish make attractive candidates for conducting stud- ies in gene expression, regulation, function, and genetic modification, since they reach sexual maturity rela- tively quickly, are highly fecund, utilize an external fertilization strategy, develop exte…
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| 关 键 词： | 输异基因 鱼 生物技术 性腺 脊椎动物 RNA |
| 收稿时间： | 2005-07-05 |
A perspective on fish gonad manipulation for biotechnical applications |
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| Wei Hu,Yaping Wang,Zuoyan Zhu. A perspective on fish gonad manipulation for biotechnical applications[J]. Chinese science bulletin, 2006, 51(1): 1-7. DOI: 10.1007/s11434-005-1055-3 |
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| Authors: | Wei Hu Yaping Wang Zuoyan Zhu |
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| Affiliation: | (1) State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan, 430072, China |
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| Abstract: | The gonad is an essential organ for gen- erating sperm and ova in vertebrates. This review describes several pilot studies on gonad gene manipulation and development in fish. With antisense RNA techniques, we suppressed the gonad devel- opment, and thus the fertility, of an antisense gonad- otropin-releasing hormone (sGnRH) transgenic common carp. Then, using a tissue-specific exoge- nous gene excision strategy with sexual compensa- tion, we knocked out the gonad-specific transgene. Under the control of the rainbow trout protamine promoter, the transgenic fish expressed the reporter gene eGFP specifically in the spermary. These re- sults indicate that the fish gonad is a new model or- gan that can improve contemporary biotechnology experiments. Herein we discuss the potential of fish gonad manipulation for resolving important biosafety problems regarding transgenic fish generation and producing the new transgenic animal bioreactor. |
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| Keywords: | fish transgene gonad manipulation. |
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