基于响应面设计的头孢他啶母液冷冻浓缩工艺优化

以头孢他啶母液为原料，采用悬浮结晶冷冻浓缩技术回收头孢他啶母液中的有效成分。在单因素试验数据基础上，以头孢他啶回收率为评价指标，应用Box-Behnken响应面设计对冷冻温度、冷冻时间以及搅拌速率3个提取条件进行3因素3水平回归分析，优化最佳提取条件。得出头孢他啶最佳回收工艺条件为：冷冻温度－7 ℃，冷冻时间3 h，搅拌速率20 r/min。验证实验结果表明，此条件下头孢他啶回收率达到93.15%。结合生产实际情况优选的悬浮结晶冷冻浓缩工艺稳定，为头孢他啶的后续研究提供了一定的参考。

Optimization of the freeze concentration process of ceftazidime mother liquor based on the response surface method

Using ceftazidime mother liquor as the raw material, we recovered its effective components using suspension freeze concentration technology. Based on single factor test data, taking the recovery rate of ceftazidime as the evaluation index, we used the Box-Behnken response surface method to analyze the three extraction conditions of freezing temperature, freezing time, and stirring rate in three levels respectively to optimize the extraction conditions. The optimum recovery conditions of ceftazidime were as follows: freezing temperature -7 ℃, freezing time 3 h, stirring rate 20 r/min. Results show that the recovery of ceftazidime under these conditions was 93.15%.Combined with the actual production situation, the optimized suspension freeze concentration process is stable and can shed light on the follow-up study of ceftazidime.