不同产地桑叶中黄酮类成分含量分析

应用高效液相色谱法检测全国不同地区采购的22批次桑叶中6种黄酮类化合物的含量，并进行精密度、稳定性、重复性和加样回收率试验以及含量试验。结果显示：精密度试验、稳定性试验检测色谱峰面积的相对标准偏差均低于1%，表明检测仪器与操作符合精密度的要求；不同产地桑叶中6种黄酮类成分的含量差异较大，其中，安徽亳州、四川绵阳、广西玉林地区采集的桑叶中黄酮总量较高，其次是河南商丘、浙江桐乡、江苏泰兴、湖北襄阳，而湖南邵阳桑叶的黄酮总量最少，提示不同产地桑叶中黄酮的含量有差异。采用高效液相色谱法测定桑叶中黄酮类成分的含量操作简便、稳定性及重复性较好。

Analysis of flavonoids in mulberry leaves fromdifferent production areas

In this study, high performance liquidchromatography was performed to detect the content of six flavonoids in 22batches of mulberry leaves purchased from different regions of the country.Precision, stability, repeatability, sample recovery rate, and flavonoidcontent were measured to determine the types and contents of flavonoids inmulberry leaves from different regions. The results showed that the relativestandard deviation (RSD) of the chromatographic peak area detected by precisionand stability tests were both <1%， this indicates that the detection instrument and operation meet theprecision requirements. The amount of flavonoids considerably varied inmulberry leaves from different regions. Among them, the total amount offlavonoids in mulberry leaves collected from Bozhou, Anhui; Mianyang, Sichuan;and Yulin, Guangxi was high, followed by that in leaves collected fromShangqiu, Henan; Tongxiang, Zhejiang; Taixing, Jiangsu; and Xiangyang, Hubei.The total amount of flavonoids in mulberry leaves collected from Shaoyang,Hunan was the lowest. The results suggest that the content of flavonoids inmulberry leaves from different regions is different. The determination of thecontent of flavonoids in mulberry leaves via high performance liquidchromatography is easy to operate, stable, and repeatable.