细茎石斛组织培养研究

以细茎石斛(Dendrobium moniliforme(L)sw.)的种子进行离体培养,获得无菌苗.截取无菌苗的茎段作为外植体,进行组织培养.结果表明:最适宜诱导细茎石斛原球茎生成的培养为MS 0.5 mg/L6-BA 2 mg/L NAA 10%CM;最适宜细茎石斛原球茎增殖及幼苗分化的培养基为MS 4 mg/L6-BA 1 mg/L KT 1 mg/L NAA 10%马铃薯浸汁;最适宜细茎石斛壮苗生根的培养基为MS 2 mg/L NAA 10%香蕉汁;最适宜细茎石斛瓶苗移栽的基质为纯苔藓.

Tissue Culture of Dendrobium Moniliforme

The research got the aseptic seedling by isolated culture of the seeds of Dendrobium moniliforme,and then conducted tissue culture on the stalk cut from the aseptic and used as explants.The result indicated that,the culture medium most suitable for the production of PLB (Protocorm-liked body) on Dendrobium moniliforme was MS 0.5 mg/L 6-BA 2 mg/LNAA 10% CM;the culture medium most appropriate for the seedling differentiation and the breeding of PLB was MS 4 mg/L6-BA 1 mg/LKT 1 mg/LNAA 10% tomato-soaking liquid;the culture medium most suitable for the rooting of sturdy seedling was:MS 2 mg/LNAA 10% banana juice;the medium favorable for the transplanting of Dendrobium moniliforme seedling was pure moss.