应用基因组原位杂交鉴定杂交后代中的簇毛麦染色体

用地高辛（Digoxigenin-11-dTup)标记的簇毛麦染色体组DNA为探针，以普通小麦“中国春”总DNA作封阻进行基因组荧光原位杂交，对小麦和簇毛麦杂交和回交后代进行检测。结果显示，在杂交后代的28条染色体中有7条簇毛麦染色体，在发生部分染色体加倍的回交代后鉴定出含7条簇毛麦染色体重的易位系。GISH的准确鉴定以及易位系的获得为向小麦导入簇志麦的有用基因提供了宝贵材料。

Identificaion of Haynaldia villosa Chromosome in Hybrids by Genomic in Situ Hybridization

Detection of the haynaldia villosa chromosomes in hybrids between common wheat and haynaldia villosa was undertaken using genomic fluorescent in situ hybridization(GISH). Digoxigenin-11-dTup labeled total genomic DNA of haynaldia villosa was used as the probe and the unabelled total DNA of commom wheat Chinese Spring(CS) as blocking DNA. The results from GISH revealed that there are 7 haynaldia villosa chromosomes in F 1 hybrids and backcross plants, translocation has taken place between one wheat chromosome and one H.villosa chrimosome.In one backcross plant, in which some wheat chromosomes reduplicated. These materials will be useful in transferring the valuable genes in H.villosa to wheat