子宫颈癌组织p16基因突变及其蛋白表达研究

目的研究子宫颈癌患者p16蛋白表达和p16基因缺失突变及点突变情况.方法利用免疫组织化学方法(SP法)、聚合酶链反应(PCR)和聚合酶链反应-单链构象多态性(PCR-SSCP)分析技术,分别检测正常子宫颈组织30例、子宫颈癌前病变组织10例及原发性子宫颈癌组织28例,观察其p16蛋白表达和p16基因缺失突变及点突变状况.结果 1)在原发性子宫颈癌组织中为67.85%(19/28),明显低于正常子宫颈组织和子宫颈癌前病变组织(P<0.05);2)28例原发性子宫颈癌组织中有11例发生p16基因缺失突变,2例发生p16基因点突变,突变率为46.42%,正常子宫颈组织和子宫颈癌前病变组织未发现p16基因缺失突变和点突变.结论 1)p16蛋白缺乏与子宫颈细胞增殖失控及分化不良紧密相关.2)原发性子宫颈癌存在p16基因点突变,以低分化癌多见,但不是较频繁的事件;原发性子宫颈癌存在p16基因缺失突变,以低分化癌多见,是较频繁的事件.3)未发现p16蛋白表达与p16基因突变有相关性.

Expression of p16 Proteins and p16 Gene Deletion Mutation and Point Mutation in Primary Cervix Carcinoma

ObjectiveTo investigate expression of p16 proteins and p16 gene deletion mutation and point mutation in primary cervix carcinoma.MethodsStreptavidin-peroxidase conjugated method (SP) was performed for detecting the expression of p16 proteins in 30 cases of normal cervix, 10 cases of the anterior lesion of cervix cancer and 28 cases of primary cervix carcinoma, and polymerase chain reaction(PCR)and polymerase chain reaction single-strand conformation polymorphism analysis (PCR-SSCP) for p16 gene deletion mutation and point mutation.Results1)The negative rate of p16 protein is (67.85%)(19/28) in primary cervix cancer, much lower than that in normal cervix and the precancerous lesion of cervix(P<(0.05)). 2)There are 11 cases of loss mutation and 2 cases of point mutation in 28 cases of primary cervix carcinoma, and the rate of mutation is (46.42%). There is not loss mutation and point mutation found in normal cervix and the precancerous lesion of cervix cancer.Conclusion1)There is a close correlation between the absence of p16 protein and cervix cell proliferation miss control and dysplasia. 2)The point mutation of p16 gene and p16 gene deletion mutation are found in primary cervix cancer, mainly in the poorly-differentiated cancer, but the former is a less frequency than the later. 3)It is not found correlation between expression of p16 protein and p16 gene mutation.