| SDS-PAGE法检测人甲状腺及其肿瘤组织中bFGFs条件的选择 |
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| 引用本文： | 陈慧敏,李尧华,刘玉和,陈奕桦.SDS-PAGE法检测人甲状腺及其肿瘤组织中bFGFs条件的选择[J].北华大学学报(自然科学版),1998(3). |
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| 作者姓名： | 陈慧敏 李尧华 刘玉和 陈奕桦 |
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| 作者单位： | 吉林医学院附属医院内科!吉林132001(陈慧敏)，吉林医学院生化教研室(李尧华)，吉林医学院病理学教研室(刘玉和)，吉林省卫生防疫站(陈奕桦) |
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| 基金项目： | 吉林省卫生厅青年科学基金 |
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| 摘 要： | 目的：探讨SDS－PAGE法检测人甲状腺及其肿瘤组织中bFGFs（basicFibroblastGrowthFactors）的条件。方法：采用SDS-PAGE电泳法检测人甲状腺及其肿瘤组织中的bFGFs．结果：分离胶浓度为12．5％，胶厚为0．5cm，电流强度为30mA，样品量为5－6ul，标准bFGFs上样量为20ul较适全人甲状腺及其肿瘤组织中各种分子量的bFGFs的分离及鉴定。结论：人甲状腺及其肿瘤组织中各种分子量的bFGFs的分离及鉴定与SDS－PAGE电泳时条件的选择密切相关。
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| 关 键 词： | SDS－PAGE 人正常甲状腺 人甲状腺肿瘤 bFGFs |
The study on the experimental condition for detecting bFGFs in human thyroid gland and its tumour tissue by means of SDS-PAGE |
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| Chen Huimin, Li Yao hua, Lui Yu he, Che Yihua.The study on the experimental condition for detecting bFGFs in human thyroid gland and its tumour tissue by means of SDS-PAGE[J].Journal of Beihua University(Natural Science),1998(3). |
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| Authors: | Chen Huimin Li Yao hua Lui Yu he Che Yihua |
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| Abstract: | Objective: To study the experimental condition for detecting bFGFs in human thyroid gland and its tumour tissue by means of SDS - PAGE. Method: To examine bFGFs in human thyroid gland and its tumour tissue by means of SDS - PAGE. Results: The best conditions for separating and identifying various molecular weight bFGFs in human thyroid gland and its tumour tissue were as follow: 12. 5% concentratcon, 0. 5cm thickness of separating gum, 30mA current intestiy , 5 - 6ul sample. 20ul marked bFGFs. Conclusion: The separation and identification of bFGFs if human thyroid gland and its tumour tissue were closely related to the experimental condition for SDS - PAGE. |
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| Keywords: | SDS-PAGE human thyroid gland human thyroid tumour bFGFs |
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