| 白芷细胞外CaM结合位点的扫描电镜定位研究 |
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| 引用本文： | 宋春凤,孙大业.白芷细胞外CaM结合位点的扫描电镜定位研究[J].河北师范大学学报(自然科学版),1998,22(4):543-546. |
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| 作者姓名： | 宋春凤 孙大业 |
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| 作者单位： | 河北医科大学电镜实验中心，河北师范大学生物系 |
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| 摘 要： | 利用胶体金标记的具有生物活性的钙调素（Ｃａｌｍｏｄｕｌｉｎ，ＣａＭ），对白芷愈伤组织培养细胞胞外钙调素结合位点进行了扫描电镜定位，发现白芷愈伤组织培养细胞表面有不同密度的金颗粒，证明其细胞表面存在着ＣａＭ结合位点．
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| 关 键 词： | 白芷 细胞外CaM 胶体金 扫描电镜 |
Studies of Extracellular Calmodulin Binding Sites on Culture Cells of Anglica Dahurica with Colloidal Gold Labelled Calmodulin by Scanning Electron Microscopy |
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| Song Chunfeng,Sun Daye,Liu Guisheng,Li Xiangyin,Guo Yi,Wang Chaohui.Studies of Extracellular Calmodulin Binding Sites on Culture Cells of Anglica Dahurica with Colloidal Gold Labelled Calmodulin by Scanning Electron Microscopy[J].Journal of Hebei Normal University,1998,22(4):543-546. |
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| Authors: | Song Chunfeng Sun Daye Liu Guisheng Li Xiangyin Guo Yi Wang Chaohui |
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| Institution: | Song Chunfeng 1) Sun Daye 2) Liu Guisheng 1) Li Xiangyin 1) Guo Yi 2) Wang Chaohui 2) |
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| Abstract: | A biologically active probe of plant calmodulin(CaM)has been prepaired with colloidal gold labelled technique,and has made a method to localize extracellular calmodulin binding sites on culture cells of Angella dahurica callus by scanning electron microscopy.The results show that there were different denty gold particles distributing on the surface of the cell wall.It was indicated that there were CaM binding sites on the cell wall. |
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| Keywords: | Angelica dahurica extracellular calmodulin colloidal gold scanning electron microscopy |
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