| 中性蛋白酶抗噬菌体生产菌株KRP406的选育 |
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| 引用本文： | 刘士清,罗健强.中性蛋白酶抗噬菌体生产菌株KRP406的选育[J].云南大学学报(自然科学版),1992,14(1):80-87. |
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| 作者姓名： | 刘士清 罗健强 |
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| 作者单位： | 云南省微生物研究所
(刘士清，董家灿)，云南省陆良酶制剂厂
(罗健强)，云南省陆良酶制剂厂(常小海) |
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| 摘 要： | 从中性蛋白酶产生菌枯草芽孢杆菌ys94菌株出发,用混合噬菌体选育出KRP406抗噬菌体变异株.在摇瓶条件下,结果表明,KRP406抗株抗多种噬菌体和不同的混合噬菌体,噬菌体从异常发酵液等获得与制备.在同时间的工业生产中,KRP406抗株的平均酶活力655lu/ml,此酶活超过原生产菌株ys94产生的酶活587u/ml.
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| 关 键 词： | 中性蛋白酶 噬菌体 抗噬菌体菌株 |
Selection of the Neutral-protease Producer, Phage-resistant Strain KRP406 |
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| Liu Shiqing Dong Jiachan.Selection of the Neutral-protease Producer, Phage-resistant Strain KRP406[J].Journal of Yunnan University(Natural Sciences),1992,14(1):80-87. |
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| Authors: | Liu Shiqing Dong Jiachan |
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| Institution: | Liu Shiqing Dong Jiachan
( Yunnan Institute of Microbiology)
Luo Jiangqiang Chang Xiaohai
( Louling Enzyme Preparation Plant. Yunnan) |
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| Abstract: | The phage-resistant variant KRP406 strain was selected and bred by phage-mixture from the neutral-protease producer, phage sensistive strain Bacillus subtilis ys94. The result showed that the strain KRP406 resisted varrious phages and different phage-mixture obtained and prepared from abnomal fermented liquor and other sources, under the condition of shaking flask and at the industrial production level. The average neutral-protease activity of the phage-resistant strain KRP406 was 6551 u / ml. It was 587u / ml over and above the original neutral-protease producer ys94 did. |
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| Keywords: | neutral-protease bacteriophage phage-resistant strain Bacillus subtilis |
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