一株耐盐细菌的鉴定及其耐盐性的初步研究

从皖北砀山盐碱地中分离到一株耐盐细菌，该菌在肉汤培养基上可耐盐至7％，在基本培养基上可耐至3％．革兰氏阳性杆菌，具芽孢，芽孢表面有明显纵状突起。孢囊膨大，周生鞭毛，接触酶阳性，65℃不生长，可以葡萄糖为底物产酸产气，G C含量为52．7mol％，根据以上特性，检索鉴定该菌为浸麻芽孢杆菌(B．macerans)并对其质粒进行了提取和消除实验，证明质粒大小约为t3Kb。耐盐性状与质粒关系不大．同时对菌体裂解物作了SDS—PAGE凝胶电泳，证实在高盐(5％)情况下比低盐(0．5％)情况下的电泳图谱多出一条蛋白质带．此外还研究了耐盐性与培养基成分之间的关系．

IDENTIFICATION OF A HALOTOLERANT BACTERIA AND PRELIMINARY STUDIES ON ITS SALT TOLERANCE

A halotolerant bacteria was isolated from the salt soda soil in the northern part of Anhui Province. The bacteria can grow well in Broth medium containing 0.5%-7% NaCl and in minimal medium containing 0.5%-3% NaCl. It is a Gram postive bacteria. In the surface of its spore, there are apparent vertical spine shaped structure. It also can utilize gluose to produce acid and gas, The G+C content is 52.7mol%. The Strain is identified as B.macerans. The organism contained a 13kb plasmid. Meanwhile we analysed the intracelleular protein under different growing conditions by SDS-polyacrylamide gel electrophoresis.This paper also explores the relation of salt tolerance and the composition of the medium.