| 植物乳杆菌产乳酸脱氢酶的发酵条件优化及酶的纯化 |
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| 引用本文： | 苏虹,汪建军,杨海麟,王武. 植物乳杆菌产乳酸脱氢酶的发酵条件优化及酶的纯化[J]. 吉林大学学报(理学版), 2006, 44(6): 1015-1018 |
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| 作者姓名： | 苏虹 汪建军 杨海麟 王武 |
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| 作者单位： | 吉林大学,药学院,长春,130021;江南大学,工业生物技术教育部重点实验室,江苏省,无锡,214036 |
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| 摘 要： | 对植物乳杆菌突变株RS2 2的发酵条件做了优化， 在25 L发酵罐上进行放大实验， 使菌株RS2 2的乳酸脱氢酶（Lactate Dehydrogenase）产酶水平有了较大提高， 细胞抽提液比酶活达到24.6U/mg．利用DEAE离子交换、 疏水层析对酶的纯化进行了研究， 最终酶的纯化倍数为49.1， 比活力达1 208.6 U/mg，初步确定了LDH的纯化路线.
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| 关 键 词： | 乳酸脱氢酶 植物乳杆菌 发酵 纯化 |
| 文章编号： | 1671-5489(2006)06-1015-04 |
| 收稿时间： | 2006-01-23 |
| 修稿时间： | 2006-01-23 |
Fermentation Optimization and Purification of Lactate Dehydrogenase from Lactobacillus Plantarum |
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| SU Hong,WANG Jian-jun,YANG Hai-lin,WANG Wu. Fermentation Optimization and Purification of Lactate Dehydrogenase from Lactobacillus Plantarum[J]. Journal of Jilin University: Sci Ed, 2006, 44(6): 1015-1018 |
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| Authors: | SU Hong WANG Jian-jun YANG Hai-lin WANG Wu |
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| Affiliation: | 1.School of Pharmaceutical Science, Jilin University, Changchun 130021, China; 2.Key Laboratory of Industry Biotechnology of Ministry of Education, Southern Yangtze University, Wuxi 214036, Jiangsu Province, China |
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| Abstract: | High output of the lactate dehydrogenase(LDH) from lactobacillus plantarum RS2-2 was achieved after optimization of the fermentation conditions and magnified experiment was carried out on 25 L fermentor.The LDH activity of cell extraction reached to (24.6 U/mg.) Purification of the LDH was also studied by means of DEAE ion-exchange and hydrophobic chromatography,which resulted in(49.1)-fold purification and(1 208.6 U/mg) specific activity.The routine of LDH separation was primarily determined. |
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| Keywords: | lactate dehydrogenase lactobacillus plantarum fermentation purification |
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