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高效液相色谱法测定奶粉中的β-胡萝卜素
引用本文:张晓慧,郭少平,陶通. 高效液相色谱法测定奶粉中的β-胡萝卜素[J]. 高师理科学刊, 1997, 0(4)
作者姓名:张晓慧  郭少平  陶通
作者单位:齐齐哈尔轻工学院(张晓慧),齐齐哈尔市电石厂(郭少平),齐市化工研究所(陶通)
摘    要:采用高效液相色谱法测定奶粉中的β-胡萝卜素.奶粉样品在甲醇溶剂中碱化后,用石油醚萃取β-胡萝卜素,经Varian30cm×4mm硅胶柱分离,紫外检测定量.该方法最低检出限为5.38×10-10g,回收率为95.3%~98.4%,变异系数为2.04%.

关 键 词:β-胡萝卜素  定量分析  奶粉  高效液相色谱法

Determination of β-Carotene in Milk Powder with HPLC
Zhang Xiaohui. Determination of β-Carotene in Milk Powder with HPLC[J]. Journal of Science of Teachers'College and University, 1997, 0(4)
Authors:Zhang Xiaohui
Affiliation:Zhang Xiaohui(Light Industry Institute of Qiqihar University)Guo Shaoping(Qiqihar Calcium Carbide Plant)Tao Tong(Qiqihar Chemical Industry Institute)
Abstract:This method is for determining β-carotene in milk powder with HPLC.The sample was saponificated in basic methanol conditions and β-carotene in the sample could be extracted by using petroleum ether,the β-carotene separated with other consistants in varian 30 cm× 4 mm Si -5 column and detested at uv453 nm. The minimum detestable value is 5. 38 ×10-10g. The variation coefficient cv (%) is 2.04. The revovery is 95. 3%~98. 4%.
Keywords:Carotene Quantitative analysis Milk powder HPLC  
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