| 高效液相色谱法测定奶粉中的β-胡萝卜素 |
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| 引用本文： | 张晓慧,郭少平,陶通. 高效液相色谱法测定奶粉中的β-胡萝卜素[J]. 高师理科学刊, 1997, 0(4) |
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| 作者姓名： | 张晓慧 郭少平 陶通 |
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| 作者单位： | 齐齐哈尔轻工学院(张晓慧)，齐齐哈尔市电石厂(郭少平)，齐市化工研究所(陶通) |
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| 摘 要： | 采用高效液相色谱法测定奶粉中的β－胡萝卜素．奶粉样品在甲醇溶剂中碱化后，用石油醚萃取β－胡萝卜素，经Varian30cm×4mm硅胶柱分离，紫外检测定量．该方法最低检出限为5．38×10－10g，回收率为95．3％～98．4％，变异系数为2．04％．
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| 关 键 词： | β-胡萝卜素 定量分析 奶粉 高效液相色谱法 |
Determination of β-Carotene in Milk Powder with HPLC |
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| Zhang Xiaohui. Determination of β-Carotene in Milk Powder with HPLC[J]. Journal of Science of Teachers＇College and University, 1997, 0(4) |
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| Authors: | Zhang Xiaohui |
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| Affiliation: | Zhang Xiaohui(Light Industry Institute of Qiqihar University)Guo Shaoping(Qiqihar Calcium Carbide Plant)Tao Tong(Qiqihar Chemical Industry Institute) |
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| Abstract: | This method is for determining β-carotene in milk powder with HPLC.The sample was saponificated in basic methanol conditions and β-carotene in the sample could be extracted by using petroleum ether,the β-carotene separated with other consistants in varian 30 cm× 4 mm Si -5 column and detested at uv453 nm. The minimum detestable value is 5. 38 ×10-10g. The variation coefficient cv (%) is 2.04. The revovery is 95. 3%~98. 4%. |
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| Keywords: | Carotene Quantitative analysis Milk powder HPLC |
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