| 淘米水发酵物中2种有机酸的测定 |
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| 引用本文： | 杜刚,陆小凯,刘仙花,洪耀强,杨海英. 淘米水发酵物中2种有机酸的测定[J]. 云南民族大学学报(自然科学版), 2019, 0(2): 114-117 |
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| 作者姓名： | 杜刚 陆小凯 刘仙花 洪耀强 杨海英 |
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| 作者单位： | 云南民族大学民族药资源化学国家民族事务委员会-教育部重点实验室;云南民族大学化学与环境学院 |
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| 摘 要： | 采用高效液相色谱法分析测定淘米水发酵液中的有机酸,确定了淘米水发酵液中有机酸分析测定的色谱条件,采用Agilent Zorbax SB-Aq(4.6 mm×150 mm,5μm)色谱柱,流动相为1%甲醇-99%NaH_2PO_4溶液(20 mmol/L,pH=2.0);紫外检测波长210 nm;流速0.4 mL/min;柱温25℃.结果表明,在该条件下淘米水发酵液中的有机酸得到了较好分离,分离检测出乳酸、乙酸2种有机酸.该分析方法简单准确,适用于淘米水发酵液中有机酸的测定分析.
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| 关 键 词： | 淘米水发酵液 乳酸 乙酸 高效液相色谱法 |
Determination of organic acids in fermented rice-washing water |
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| Affiliation: | ,Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission and Ministry of Education,School of Chemistry and Environment, Yunnan Minzu University |
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| Abstract: | A HPLC(high performance liquid chromatography) method was developed for the determination of organic acids in fermented rice-washing water. HPLC separation was carried out in a Zorbax SB-Aq column(4.6 mm×150 mm,5 μm) with methanol-20 mmol/L NaH_2PO_4 solution(1∶99, V/V) as the mobile phase. The velocity was 0.4 mL/min, the column temperature was 25 ℃, and the detection wave length was 210 nm. The results showed that the lactic acid and acetic acid in the fermented rice-washing water were separated and determined. This simple and accurate method could be used for the determination of organic acids in the fermented rice-washing water. |
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| Keywords: | fermented rice-washing water lactic acid acetic acid HPLC |
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