| 带有XylE基因的EB病毒穿梭质粒pFD891的构建 |
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| 引用本文： | 王晓利,戴一凡,邱信芳,薛京伦.带有XylE基因的EB病毒穿梭质粒pFD891的构建[J].复旦学报(自然科学版),1990(4). |
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| 作者姓名： | 王晓利 戴一凡 邱信芳 薛京伦 |
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| 作者单位： | 复旦大学遗传学研究所
(王晓利，戴一凡，邱信芳)，复旦大学遗传学研究所(薛京伦) |
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| 摘 要： | 构建了一个以EB病毒为基础、以邻苯二酚氧化酶(XylE)基因为靶基因、以潮霉素B邻酸转移酶为转化细胞的选择标记基因的穿梭质粒。这种质粒既能在细菌中复制,又能在真核细胞中自主复制,并有较高的拷贝数,因而能很容易地从真核细胞中分离出来,并可用来研究基因突变和突变类型的形成机制,建立一种化学物质诱变性的分子检测系统。
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| 关 键 词： | EB病毒 质粒 基因突变 邻苯二酚氧化酶基因 穿梭质粒 |
THE CONSTRUCTION OF AN EB VIRUS BASED SHUTTLE VECTOR pFD891 CONTAINING Xy1E GENE |
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| Wang Xiaoli,Dai Yifan,Qiu Xinfang,Xue Jinglun,.THE CONSTRUCTION OF AN EB VIRUS BASED SHUTTLE VECTOR pFD891 CONTAINING Xy1E GENE[J].Journal of Fudan University(Natural Science),1990(4). |
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| Authors: | Wang Xiaoli Dai Yifan Qiu Xinfang Xue Jinglun |
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| Institution: | Institute of Genetics |
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| Abstract: | An EB virus based shuttle vector pFD891 was costructed, carrying the targetgene catechol 2, 3-dioxygenase gene (XylE) and hygromycin B phosphotransferasegene (hyg~r) as the selectable marker gene for transformed cells. The vector repli-cate not only in bacteria, but also autonomously in eukaryotic ceils. It can be easilyisolated from the mammalian cells because of its high copies. The pFD891 shuttlevector could be used in studying the mechanism and types of mutation; and in theestablishment of a system for detecting the mutagenicity of potential mutagens andcarcinogens. |
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| Keywords: | Epstein-barr virus plasmid gene mutatin XylE gene shuttle vector |
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