等离子体浸没离子注入豌豆种子对过氧化物酶同工酶的影响

用等离子体浸没离子注入设备对豌豆种子进行N~+注入.用聚丙烯酰胺凝胶电泳技术对豌豆胚芽中的过氧化物酶同工酶进行分离,用联苯胺染色法进行显色.发现过氧化物酶同工酶的活性和数量随离子注入剂量的变化而呈明显变化.酶谱分析可知,注入离子影响了过氧化物酶同工酶酶谱和迁移率.说明离子注入对生物体的影响可在同工酶水平上得到反映.从一个侧面证实了注入离子与生物酶分子之间存在质量沉积和电荷交换效应.

Study on the Changes of the POD Isozyme in Pea Seed with Plasma Immersion Ion Implantation

The pea seeds are implanted with Nitrogen ions by plasma immersion ion implantation. The POD isozyme in pea plumule is separated by gel electrophoresis method. It is shown that the activity and the mass deposition of the POD isozyme in pea plumule vary as implantation dose changed. The patterns of gel electrophoresis of the POD isozyme reveal that implanted ions affect the structure and the electric character of isozyme molecules. It is proved that the effect of ion implantation has come up to biochemistry level. On the side, it is shown that quality deposition and charge exchange exist between the implanted ions and isozyme molecules.