(1) State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072 Wuhan, China
Abstract:
Single later blastula nuclei from AB strain of zebrafish (Danio rerio) were transplanted into enucleated unfertilized eggs of Long fin strain. Of 1119 cloning embryos, 14 reconstructed embryos
developed into fry. DNA fingerprinting systems of the cloned fish were similar to those of the nuclear donor fish, but were
distinctly different from those of the nuclear recipient fish. It confirmed that the genetic material originated from nuclear
donor cell other than from nuclear recipient egg. The research suggested that the basic technique for nuclear transplantation
performed with different strains of zebrafish has made a breakthrough. It should be helpful for the study of some important
developmental problems such as gene function, the regulation ogene expression during animal development, the developmental
potential of a nucleus and the interactions between the donor nucleus and the recipient cytoplasm, etc.