| 甘薯羽状斑驳病毒中国分离株外壳蛋白基因的克隆和序列分析 |
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| 引用本文： | 孟清,温利华,王凤武,金萍,孟学平,李霞.甘薯羽状斑驳病毒中国分离株外壳蛋白基因的克隆和序列分析[J].内蒙古大学学报(自然科学版),2005,36(1):68-74. |
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| 作者姓名： | 孟清 温利华 王凤武 金萍 孟学平 李霞 |
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| 作者单位： | 内蒙古大学生物系,呼和浩特,010021;淮海工学院海洋学院,江苏,连云港,222005 |
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| 基金项目： | 国家自然科学基金资助项目(39460047) |
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| 摘 要： | 根据已报道的甘薯羽状斑驳病毒(Sweetpotatofeatherymottlevirus,SPFMV)外壳蛋白基因序列,设计合成了一对特异性引物,以我们从国内甘薯品种徐薯-18上分离到的SPFMV的RNA为模板,经过反转录合成cDNA第一条链,经PCR扩增、限制性酶切后克隆于pUC19的Hind 和SacI位点,转化大肠杆菌JM109,经限制性酶切分析、PCR鉴定以及序列分析证实获得了SPFMV中国分离株外壳蛋白基因的全长克隆.
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| 关 键 词： | 甘薯羽状斑驳病毒 外壳蛋白基因 基因克隆 |
| 文章编号： | 1000-1638(2005)01-0068-07 |
| 修稿时间： | 2004年8月9日 |
Cloning and Sequence Analysis of Coat Protein Gene of Sweet Potato Feathery Mottle Virus Chinese Isolate (SPFMV-Ch) |
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| MENG Qing,WEN Li-hua,WANG Feng-wu,JIN Ping,MENG Xue-ping,LI Xia.Cloning and Sequence Analysis of Coat Protein Gene of Sweet Potato Feathery Mottle Virus Chinese Isolate (SPFMV-Ch)[J].Acta Scientiarum Naturalium Universitatis Neimongol,2005,36(1):68-74. |
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| Authors: | MENG Qing WEN Li-hua WANG Feng-wu JIN Ping MENG Xue-ping LI Xia |
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| Institution: | MENG Qing~1,WEN Li-hua~1,WANG Feng-wu~1,JIN Ping~1,MENG Xue-ping~2,LI Xia~1 |
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| Abstract: | The cDNA of SPFMV coat protein gene was synthesized by RT-PCRwith artifically synthesized two specific primers and the RNA of sweet potato feathery mottle virus Chinese isolate as templete. The synthesized cDNA was cloned into plasmid pUC19 in E. coli JM109. The recombinant plasmid was identified by PCR, restriction endonuclease and sequence analysis.The tests show that the coat protein gene of SPFMV-Ch was obtained. |
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| Keywords: | sweet potato feathery mottle virus coat protein gene gene cloning |
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