| 虎眼万年青的体细胞胚胎直接发生与植株再生 |
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| 引用本文： | 张光祥 鲍晓明. 虎眼万年青的体细胞胚胎直接发生与植株再生[J]. 四川师范大学学报(自然科学版), 1994, 17(3): 53-57 |
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| 作者姓名： | 张光祥 鲍晓明 |
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| 作者单位： | 四川师范大学生物系，东北师范大学遗传与细胞研究所 |
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| 摘 要： | 本文结果表明，在含ＮＡＡ和ＢＡ各０．５ｍｇ／ｌ的ＭＳ培养基上，体细胞胚以高频率直接发生于虎眼万年青休眠子鳞茎鳞片切段的近轴面原形态学基部一侧。体细胞胚在残余的半干旱培养基上失水处理后萌发率显著提高，并产生根系完整的健壮再生植株。
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| 关 键 词： | 虎眼万年青 体细胞胚 胚胎发生 |
DIRECT SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN ORNITHOGALUM CAUDATUM Ait. |
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| Zhang Guangxiang. DIRECT SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN ORNITHOGALUM CAUDATUM Ait.[J]. Journal of Sichuan Normal University(Natural Science), 1994, 17(3): 53-57 |
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| Authors: | Zhang Guangxiang |
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| Abstract: | general, reproducible plant-regeneration procedure for Ornithogalum caudatum is described. Direct somatic embryogenesis was induced at a high frequency from the adaxial surface of circular bulb-scale segments by culturing the explants on MS medium supplemented with 0. 5mg/1 asphthaleneacetic acid, 0. 5mg/l 6-benzyladenine and 500mg/1 casein hydrolysate. Gradual dehydration by placing the somatic embryos on residual semiarid medium showed its effect on promoting conversion to plantlet at a high rate. |
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| Keywords: | Ornithogalum caudatum Somatic embryogenesis dehydration treatment plant regeneration |
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