Effect of acetazolamide on stable carbon isotope fractionation in <Emphasis Type="Italic">Chlamydomonas reinhardtii</Emphasis> and <Emphasis Type="Italic">Chlorella vulgaris</Emphasis> |
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| Authors: | YanYou Wu Ying Xu HaiTao Li DeKe Xing |
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| Institution: | Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002, China; 2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | The effect of extracellular carbonic anhydrase (CAex) on stable carbon isotope fractionation in algae is still unclear. The
stable carbon isotope composition and algal growth in the presence and absence of the membrane-impermeable CA inhibitor acetazolamide
were compared in Chlamydomonas reinhardtii and Chlorella vulgaris. The CAex of both algal species contributed about 9‰ of the stable carbon isotope fractionation and exhibited a dosage effect.
Therefore, evidence in vivo that CAex leads to a larger carbon isotope fractionation of algae is presented. |
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| Keywords: | acetazolamide algae bicarbonate carbonic anhydrase stable carbon isotope |
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