|
|||||
|
|
| 光谱法测定植物组织中甜菜碱含量方法的改良 | |
| 引用本文： | 郭培国,宋波龙,许兰桂,郑岩松,李荣华,张华,黄红娣. 光谱法测定植物组织中甜菜碱含量方法的改良[J]. 广州大学学报(自然科学版), 2011, 10(3): 32-36 |
| 作者姓名： | 郭培国 宋波龙 许兰桂 郑岩松 李荣华 张华 黄红娣 |
| 作者单位： | 1. 广州大学植物基因功能研究广州市重点实验室,广东广州510006;广州大学生命科学学院,广东广州510006 2. 广州大学生命科学学院,广东广州,510006 3. 广州市农科院,广东广州,510308 |
| 基金项目： | 国家自然科学基金项目，广东省科技计划项目，广州市科技计划项目 |
| 摘 要： | 甜菜碱的积累水平与植物抗逆性密切相关,因此准确分析植物组织中甜菜碱含量可了解植物的抗逆能力.在总结前人研究的技术基础上,对传统的光谱法测定甜菜碱含量方法进行了一些改良,用少量乙醇溶解雷氏盐,调整雷氏盐与甜菜碱反应时间为6h.多次试验表明,该改良的光谱法能准确测定出植物组织中的甜菜碱含量. |
| 关 键 词： | 抗逆性 甜菜碱 光谱法 改良 苦瓜 |
An improved spectrophotometric method for determination of betaine content in plants |
|
| GUO Pei-guo,SONG Bo-long,XU Lan-gui,ZHENG Yan-song,LI Rong-hua,ZHANG Hua,HUANG Hong-di. An improved spectrophotometric method for determination of betaine content in plants[J]. Journal og Guangzhou University:Natural Science Edition, 2011, 10(3): 32-36 | |
| Authors: | GUO Pei-guo SONG Bo-long XU Lan-gui ZHENG Yan-song LI Rong-hua ZHANG Hua HUANG Hong-di |
| Affiliation: | GUO Pei-guo1,SONG Bo-long1b,XU Lan-gui1b,ZHENG Yan-song2,LI Rong-hua1,ZHANG Hua2,HUANG Hong-di2(1a.Guangzhou Key Laboratory for Functional Study on Stress-resistant Genes in Plants,b.School of Life Sciences,Guangzhou University,Guangzhou 510006,China,2.Guangzhou Academy for Agricultural Science,Guangzhou 510308,China) |
| Abstract: | Betainen accumulation is associated with the plant resistance to stresses,thus precise determination of betaine content in plants is helpful to understand plant resistant capability to stresses.Based on the principles,the spectrophotometric method is ameliorated to precisely determine the betaine content in plants in this study with modification procedures mainly including that the Reinecke salt is dissolved with a little ethanol,and the reaction time of Reinecke salt and betaine is optimized to 6 h.Results... |
| Keywords: | stress resistance betaine spectrophotometric method improvement Momordica charantia |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |