| 长白蝮蛇类凝血酶基因(Ussurase)的克隆及分析（英文） |
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| 引用本文： | 杜道林,邓晓东,吴文芳,吕国安,徐梅.长白蝮蛇类凝血酶基因(Ussurase)的克隆及分析（英文）[J].海南师范大学学报(自然科学版),2001,14(3):85-93. |
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| 作者姓名： | 杜道林 邓晓东 吴文芳 吕国安 徐梅 |
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| 作者单位： | 中国科学院沈阳应用生态研究所, |
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| 基金项目： | 中国科学院"九五"重点项目(KZ-952-SI-226) |
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| 摘 要： | 从长白蝮蛇（Agkistrodon halys Ussuriensis）毒腺中抽提总RNA,采用RT-PCR扩增其类凝血酶基因,经全序列测定,类凝血酶基因ussurase全长为699个核苷酸,即编码233个氨基酸;根据同源性,推测它的活性中心为His40,Asp85和Ser179;二硫键为Cys7-Cys138,Cys25-Cys41,Cys73-Cys231,Cys117-Cys185,Cys149-Cys164和Cys175-Cys200。该蛇毒类凝血酶cDNA序列及推导的氨基酸序列均为首次报道。
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| 关 键 词： | 长白蝮蛇（Agkistrodon halys Ussuriensis） 类凝血酶基因 克隆 序列分析 |
| 修稿时间： | 2001年6月13日 |
Cloning and analysis of cDNA for thrombin-like enzyme,ussurase from Agkistrodon halys ussuriensis snake venom |
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| Abstract.Cloning and analysis of cDNA for thrombin-like enzyme,ussurase from Agkistrodon halys ussuriensis snake venom[J].Journal of Hainan Normal University:Natural Science,2001,14(3):85-93. |
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| Authors: | Abstract |
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| Abstract: | Total RNA was extracted from the venom gland of the Agkistrodon halys Ussuriensis snake. The thrombin - like enzyme gene was amplified by RT -PCR, cloned, and its nucleotide sequence had been determined. The enzyme called ussurase cDNA encodes 699 nucleotides, namely 233 amino acids. Based on the ho-mology, the catalytic residues and disulfide bridges of ussurase were deduced as follows: catalytic residues, His40, Asp85 and Ser179; and disulfide bridges, Cys7-Cys138, Cys25 -Cys41, Cys73 - Cys231, Cys117 - Cys185, Cys149 - Cys164 and Cys175- Cys200. |
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| Keywords: | Agkistrodon halys Ussuriensis thrombin-like enzyme gene ussur-ase clone sequence analysis |
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