液态混合菌发酵优化纤维素酶组分

在里氏木霉和黑曲霉液态混合条件下培养纤维素酶,分析两个菌种的接种比和延迟黑曲霉的接种时间对产酶的影响,探讨两个菌种发挥协同作用的最佳条件。结果表明:黑曲霉延迟接种48 h及里氏木霉与黑曲霉接种质量比1∶1时,所产纤维素酶的滤纸酶活为1.163μmol/(min.mL);β-葡萄糖苷酶活为0.606μmol/(min.mL),β-葡萄糖苷酶活与滤纸酶活比值为0.521,比单一里氏木霉产纤维素酶的酶活高,并在后续的酶解效果对比中表现最佳。48 h时的酶解得率为65.61%,高于里氏木霉单一培养时所产纤维素酶的得率53.91%和商品纤维素酶的得率49.64%。说明通过混合发酵,纤维素酶的组分得到了优化。

Optimization of cellulase components by mixed culture of Trichoderma reesei and Aspergillus niger

Mixed culture of Trichoderma reesei and Aspergillus niger was investigated to optimize the cellulase components in liquid-state fermentation.A mode of delayed inoculation was applied for co-cultivation of T.reesei and A.niger.After cultivation of T.reesei for 48 h,A.niger was inoculated at the 1∶1 inoculum ratio.The β-glucosidase activity and filter paper activity of the mixed fungi culture were 0.606 μmol/(min·mL) and 1.163 μmol/(min·mL),respectively,corresponding to a ratio of 0.521,which was higher than that of T.reesei under single cultivation.The yield of enzymatic hydrolysis was 65.61 % by the co-cultivated cellulase,which was higher than those of single-cultivated cellulase and commercial cellulase.These results demonstrated that co-cultivation of T.reesei and A.niger was suitable for optimization of cellulase components.