| 借铝（Ⅲ）螯合物和高效液相色谱测定四环素类药物 |
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| 引用本文： | 张孝松,刘轶春,王蕾,林长山.借铝（Ⅲ）螯合物和高效液相色谱测定四环素类药物[J].中国科学技术大学学报,2001,31(2):213-217. |
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| 作者姓名： | 张孝松 刘轶春 王蕾 林长山 |
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| 作者单位： | 中国科学技术大学化学系, |
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| 摘 要： | 借离子对反相高效液相色谱分离美满霉素(MNC)、土霉素(OTC)、美他霉素
(MTC)和金霉素(CTC)的铝(Ⅲ)螯合物并于激发波长380nm和发射波长480nm处
检测.研究了螯合物的最宜分离和测定条件.于KromasilODS柱(250×4.6mm
I.D.,5μm)上，用含0.050mol/L柠檬酸缓冲剂(pH2.5)作流动相，流速为0.7mL/
min.检测限为0.12ngMNC,0.2ngOTC,1.8ngMTC和0.6ngCTC.
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| 关 键 词： | 液相色谱 四环素类药物 衍生剂Ⅲ 荧光检测 测定 螯合物 |
| 文章编号： | 0253-2778(2001)02-0213-05 |
Determination of
Tetracyclines as Chelates With Aluminium( |
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| ZHANG Xiao-song,LIU Yi-chun,WANG Lei,LIN Chang-shan.Determination of
Tetracyclines as Chelates With Aluminium([J].Journal of University of Science and Technology of China,2001,31(2):213-217. |
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| Authors: | ZHANG Xiao-song LIU Yi-chun WANG Lei LIN Chang-shan |
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| Abstract: | Minocycline(MNC), oxytetracycline(OTC), methacycline(MTC) and chlortetracy
cline(CTC) as chelates derivatized pre-column in a solution of 0.4 mol/L acetic acid-sodium
acetate buffer (pH 4.0) with aluminium( Ⅲ ) were separated by ion-pair reversed-phase high
performance liquid chromatography and detected spectrofluorimetrically with excitation wave
length at 380 nm and emssion wavelength at 480 nn. The optimum conditions for the separation
and determination of MNC-, OTC-, MTC- and CTC-AI( Ⅲ ) chelates were investigated. The
chelates were separated on a Kromasil ODS column (250× 4.6 mm I.D., 5 μn) using ace
tonitrile-dimethylfonnamide-water (5: 20: 75, V/V/V) containing 0.050 mol/L citric acid
buffer (pH 2.5) as a mobile phase at a flow rate of 0.7 mL/min. The detection limits of the
assay (signal-to-noise ratio = 3: 1) were 0.12 ng, 0.2 ng, 1.8 ng and 0.6 ng for MNC,
OTC, MTC and CTC, respectively. |
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