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| 甘蔗蔗糖磷酸合成酶基因中间缺失突变体的构建 | |
| 引用本文： | 宋喜梅,叶冰莹,林荣华,代容春,何文锦,陈由强,陈如凯.甘蔗蔗糖磷酸合成酶基因中间缺失突变体的构建[J].福建师范大学学报(自然科学版),2010,26(1). |
| 作者姓名： | 宋喜梅 叶冰莹 林荣华 代容春 何文锦 陈由强 陈如凯 |
| 作者单位： | 1. 福建师范大学生命科学学院,福建,福州,350108;农业部甘蔗生理生态与遗传改良重点开放实验室,福建,福州,350108;发育与神经生物学福建省高等学校重点实验室,福建,福州,350108 2. 福建师范大学生命科学学院,福建,福州,350108;农业部甘蔗生理生态与遗传改良重点开放实验室,福建,福州,350108 3. 农业部甘蔗生理生态与遗传改良重点开放实验室,福建,福州,350108 |
| 基金项目： | 国家自然科学基金资助项目，国家高技术研究发展计划"863"课题，福建省科技厅资助项目 |
| 摘 要： | 根据NCBI中EU278617序列设计引物,利用甘蔗SPSG2菌液为模板,扩增SPS8-11并克隆到T载体后测序.通过排除野生型DNA的定点突变PCR方法获得SPS8-11中第8内含子缺失TGCATG的突变体pMD-SPS8-11-A,酶切后测序鉴定.成功构建了甘蔗SPS8-11中间缺失突变体pMD-SPS8-11-A. |
| 关 键 词： | 蔗糖磷酸合成酶基因 定点突变 中间缺失突变体 模序 |
Construct Middle Fragment Deleted Mutant of Sucrose Phosphate Synthase Gene from Saccharum officinarum |
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| SONG Xi-mei,YE Bing-ying,LIN Rong-hua,DAI Rong-chun,HE Wen-jin,CHEN You-qiang,CHEN Ru-kai.Construct Middle Fragment Deleted Mutant of Sucrose Phosphate Synthase Gene from Saccharum officinarum[J].Journal of Fujian Teachers University(Natural Science),2010,26(1). | |
| Authors: | SONG Xi-mei YE Bing-ying LIN Rong-hua DAI Rong-chun HE Wen-jin CHEN You-qiang CHEN Ru-kai |
| Abstract: | Designed primers according to EU278617 of NCBI. Amplified the target SPSⅢ8-11 by PCR protocol from sugarcane SPSⅢG2, then cloned into pMD18-T vector and sequenced. The middle fragment deleted mutant of SPSⅢ8-11 (pMD-SPSⅢ8-11-A) was induced by PCR-based site-directed mutagenesis, digested and sequenced.Middle fragment deleted mutant of SPSⅢ8-11 from sugarcane (pMD-SPSⅢ8-11-A) was constructed successfully. |
| Keywords: | sucrose phosphate synthase gene middle fragment deletion mutant site-directed mutagenesis motifs |
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