植物激素对荞麦组织培养的研究

分别用荞麦自然苗和无菌苗的幼茎和幼叶作材料进行荞麦的组织培养,结果发现,荞麦愈伤组织诱导的最佳培养基是MS 2,4-D(7.0 mg/L) 6-BA(0.5 mg/L),幼茎的出愈率为100%,在器官诱导实验中发现MS 2,4-D(0.2 mg/L) 6-BA(4.0 mg/L)为芽诱导的最佳培养基,诱导率高达90%;根诱导最佳培养基为MS 2,4-D(4.0 mg/L) KT(0.2 mg/L),诱导率为78%.虽然荞麦自然苗和无菌苗在愈伤组织和芽诱导中无明显差异,但自然苗外殖体愈伤组织的生长和颜色与无菌苗的不同,且芽生长状况也更良好.同时,还探讨了培养基中琼脂浓度对荞麦愈伤组织诱导的影响.

Study on tissue culture of buckwheat

Stem and leaf segments of Buckwheat were cultured on MS medium supplemented with different phytohomne.The experiment results showed that the suitable phytohomne composition to induce callus was MS 2,4-D(7.0 mg/L) 6-BA0.5 mg/L),and induction rate of stem segment callus was 100%.The suitable phytohomne composition was MS 2,4-D(0.2 mg/L) 6-BA(4.0 mg/L)to induce adventitious buds and MS 2,4-D(4.0 mg/L) KT(0.2 mg/L) to induce adventitious roots.The induction rate of adventitions buds and roots was 90% and 78% respectively.Natural young plant and pollution-free young plant of Buckwheat had no sensible difference on induction to callus or organs,but the callus color of nature young plant was different form that of pollution-free young plant's callus color.Natural young plant' buds grew better than pollution-free young plant's.Meanwhile,The experiment results showed agar concentration can affect induction rate of callus.