| 蓍草组织培养的研究 |
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| 引用本文： | 庞晓斌,白杰英,王云,李彦舫,董永义.蓍草组织培养的研究[J].内蒙古民族大学学报(自然科学版),2003,18(1):34-37. |
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| 作者姓名： | 庞晓斌 白杰英 王云 李彦舫 董永义 |
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| 作者单位： | 1. 内蒙古民族大学农学院,内蒙古通辽,028042
2. 中国人民解放军军需大学植物基因工程研究中心,吉林长春,130062 |
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| 摘 要： | 以MS和8114为基本培养基，通过调整激素种类与浓度等培养条件，按正交实验设计的原则，摸索出建立蓍草组织培养体系的最佳条件：愈伤诱导最佳培养基为8114＋6BA1.0 ZT1.0；最佳分化培养基为8114 6BA4.0 NAA0.2 ZT1.0；最佳生根培养基是1/2MS0 0.1NAA。
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| 关 键 词： | 蓍草（Achillea alpina L.） 组织培养 正交设计 |
| 文章编号： | 1671-0185(2003)01-0034-04 |
| 修稿时间： | 2002年12月11 |
Study on Tissue Culture of Achillea alpina L. |
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| PANG Xiao-bin ,BAI Jie-ying ,WANG Yun ,LI Yan-fang ,DONG Yong-yi.Study on Tissue Culture of Achillea alpina L.[J].Journal of Inner Mongolia University for the Nationalities(Natural Sciences),2003,18(1):34-37. |
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| Authors: | PANG Xiao-bin BAI Jie-ying WANG Yun LI Yan-fang DONG Yong-yi |
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| Institution: | PANG Xiao-bin 1,BAI Jie-ying 2,WANG Yun 1,LI Yan-fang 2,DONG Yong-yi 1 |
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| Abstract: | In this paper,MS and 8114 were selected as the base culture medium.According to the orthogonal design principle,the optimal culture conditions of Achillea alpina L. were found through adjusting the varioties and concentration of hormones.The most appropriated culture medium in culture stages were 8114+6BA1.0+NAA1.0+ZT1.0 for inducing callus,8114+6BA4.0+NAA0.2+ZT1.0 for differentiation ,and 1/2MS0+0.1NAA for generating roots. |
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| Keywords: | Achillea alpina L Tissue culture Orthogonal design |
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