吐温-80和脂质体对厚朴酚荧光光谱的影响

分析厚朴酚在吐温-80胶束和脂质体溶液中荧光光谱的变化，发现：1）浓度为2mmol·L^-1的吐温-80胶束对厚朴酚有荧光增敏作用，厚朴酚存在于吐温-80的疏水核中，其荧光光谱发生了明显的红移；2）在4mg·mL^-1的脂质体溶液中，厚朴酚的荧光光谱变得复杂，谱图信息反映厚朴酚同时存在于水相和双脂层膜相中，且在二者之间存在分配平衡，通过测定λ365、λ397处的荧光光谱随厚朴酚浓度的变化，得到了厚朴酚在脂质体膜相和水相中的分配平衡关系；3）脂质体溶液中加入吐温-80，厚朴酚在双脂层膜相中的分布增加，其荧光光谱发生了红移．

The Effect of Tween-80 and Liposome on the Spectrum of Magnolol

Examination of the changes in spectrum of magnolol in tween-80 micel and liposome solution reveals that 1） the tween-80 micel of the density of 2 mmol·L^-1 has sensitization effect on magnolol and when magnolol is present in hydrophobic core of tween-80, its florescent spectrum undergoes obvious red shift; 2） in the liposome solution of density of 4 mg·mL^-1, the fluorescent spectrum of magnolol becomes complex, and spectrum information shows that magnolol simultaneously exists in water phase and double-layer film phase and there is a distribution balance between them and the distribution balance formula can be obtained by examining the variation of the fluorescent spectrum at λ365 and λ397; 3） when tween-80 is added to liposome solution, the distribution of magnolol in double-layer film phase increases and its fluorescent spectrum changes and becomes Complex.