短期模拟微重力对SD乳鼠海马组织细胞的效应

 为了探究模拟微重力对新生乳鼠海马脑区的影响,将新生1 d SD(Sprague Dawley)鼠的海马脑区分别做组织块培养和原代细胞培养,将培养物随机分为模拟微重力组1 d组和地面对照组。回转1 d后,用苏木精-伊红染色法(HE染色)、显微镜观察等方式检测组织块表面及内部形态变化,并用流式细胞术检测原代细胞的凋亡情况。通过明场和相差显微镜观察到与对照组相比,组织块经过1 d的模拟微重力刺激后,其厚度发生变化,组织块边缘变薄,透光度增加;HE染色结果显示,与对照组相比,模拟微重力组组织块内部细胞分布散乱。流式细胞仪检测结果显示,原代细胞在经过1 d的模拟微重力刺激后,其细胞凋亡率与对照组相比无显著差异。以上实验结果说明,经过1 d的模拟微重力后,海马组织块的表面和内部形态发生了较大变化,但凋亡率和地面对照组的相比无显著差异。

Effects of short-term simulated microgravity on hippocampal tissue blocks and primary cells of neonatal SD rats

Microgravity is one of the crucial factors in space that impact the healthy and work efficiency of astronauts. Therefore extensive research on the effects of microgravity or simulated microgravity on both human and animals has been carried out. The present study investigates whether simulated microgravity could influence the morphology and apoptosis of hippocampal tissue blocks and primary cells. Tissue blocks and primary cells are obtained from fresh hippocampus of neonatal SD rats. After being cultured for 7 days at 37℃, 95% of humidity and 5% CO2, cultures are randomly divided into a control group and a simulated microgravity group. The microgravity group is exposed to clinostat for one day and the control group is cultured on the same horizontal line of the clinostat. Then, microscope and HE staining are used to observe the surface morphology and internal morphology. In addition, apoptosis is measured by flow cytometry. As shown in the results, after treatment with 1-day simulated microgravity, the edge of tissue blocks becomes thinner and the cells within tissue blocks distribute in disorder, as compared to the control group. But apoptosis of primary cells show no difference between the simulated microgravity group and the control group. It is indicated that there is significant morphological difference of tissue blocks between the two groups after short-term simulated microgravity. However, apoptosis of primary cells seems not being affected by short-term simulated microgravity at all.