重组人白细胞介素6工程菌的表达和功能初探

自人外周血的mRNA中获得了白细胞介素6(Interleukin-6, IL-6)cDNA,基因被克隆并在大肠杆菌中表达，在20L发酵罐中每L发酵液可得60g湿菌体，IL-6的表达量约占大肠杆菌全蛋白的30%以上，占包含体蛋白的60%。纯化产物在长期，急性毒性和三致实验正常的基础上，用恒河猴的功能实验表明，在不制造血象降低模型的情况下，仍能明显增加血液中血小板和白细胞的数量。

Expression of Engineering Bacterium and Function of Recombinant Human Interleukin-6(IL-6)

Interleukin|6(IL|6) cDNA was obtained from the mRNA of human peripheral blood. Gene was cloned and expressed in %E.coli.% Sixty grams wet pellet was obtained from per liter fermentation liquid in 20L fermentation equipment. There were more than 30% IL|6 protein in the whole protein of the bacterium and 60% IL|6 protein in the inclusion body. Upon the basis of normal of long toxicity and short toxicity, the experiment on rhesus monkey shows that IL|6 can increase the quantity of platelet and leukocyte obviously without the model of decreasing hemogram.