Effect of actinomycin D onTrypanosoma cruzi |
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Authors: | M Queiroz da Cruz H M Bräscher J R Vargens A Oliveira-Lima |
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Institution: | (1) Instituto de Puericultura e Pediatria da UFRJ, and Centro de Pesquisas Arlindo de Assis da FAP, CEP, 2003 Rio de Janeiro, Brazil |
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Abstract: | Summary Viable metacyclic forms ofT. cruzi, Y strain, treated with an adequate dose of actinomycin D (50 g Act-D/ml/107 parasites/ml for 72 h at 28° C) showed the following properties: 1) they lost their ability to replicate in culture medium, in blood and in tissues of normal mice and were no longer able to incorporate tritiated thymidine; 2) they could no penetrate into Vero cells and could not replicate inside normal macrophages; 3) they retained their immunogenicity and the ability to protect mice against a virulent infection; 4) they did not induce histological lesions as described in chronic experimental Chagas' disease. |
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Keywords: | Actinomycin D Trypanosoma cruzi vaccination |
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