应用微卫星标记对海南和广西恒河猴遗传多样性的研究

目的研究微卫星DNA多态性在海南和广西恒河猴遗传多样性中的应用。方法利用11个微卫星位点,对来自这两个地区的恒河猴进行了遗传检测,并通过POPGEN32软件计算各个微卫星座位的等位基因频率、有效等位基因数目(Ne)、多态信息含量(PIC)和遗传杂合度(H)。结果所选择的11个微卫星位点均存在高度的遗传多态性,H为0.6848~0.8785;PIC为0.5826~0.8345;Ne为3.0000~7.1553。以11个微卫星标记为测度,广西恒河猴的Ne、PIC和H的平均值均高于海南猴,分别为4.2583、0.7090、0.7706(广西恒河猴)和4.2054、0.7025、0.7656(海南恒河猴)。结论这种差别可能与地域来源有关。这些研究为微卫星标记分析恒河猴遗传多样性提供理论基础。

Study on the Genetic Diversity Among Rhesus monkey from Hainan and Guangxi by Using Microsatellite Markers

Objective To analyse genetic diversity among Rhesus monkey from Hainan and Guangxi by using microsatellite DNA.Methods Eleven microsatellite loci were tested for the rhesus monkeys from Hainan and Guangxi.Allele frequency,number of effective alleles(Ne),polymorphism information contents(PIC) and heterozygosity(H) for every microsatellite locus were calculated by softwrare Popgene32.Results All eleven microsatellite loci were highly polymorphic with heterozygosity ranging from 0.7066 to 0.9025,polymorphism information content from 0.5143 to 0.8270 and number of effective alleles from 3.0000 to 7.1553.Mean values of Ne、PIC and H for Guangxi rhesus monkeys were a little higher than those for Hainan respectively.Conclusion The differences could be attributed to the different geographic origins of two populations.This study laid out a theoretical foundation for further analysis of the genetic diversity among rhesus monkeys by using microsatellite markers.